Department of Medical Biochemistry, Recep Tayyip Erdoğn University, Rize, Turkey.
Turk J Med Sci. 2014;44(1):42-9.
To analyze the phenolic composition of the Cimin grape, which is 1 of 2 grape varieties with a protected geographical indication in Turkey and is used locally to treat the symptoms of some disorders such as impotence and cardiovascular diseases, and to investigate its antioxidant potency against oxidant mediators in the models of intra- and extracellular surroundings.
Cimin grape samples were classified into 5 groups according to the grape's tissues and the extraction solvents used. Free radical scavenging (DPPH) and antilipid peroxidation product [thiobarbituric acid reactive substance (TBARS) and conjugated diene (CD)] levels of the grape tissues were extrapolated from the measurement of total phenolic and individual monomeric flavanol contents in each extract.
The seed methanolic extract had the highest total amount of flavanols, with the amount of catechin at 4.034 mM. The DPPH activities of the seed extracts were 2- to 10-fold higher than those of the other samples. The seed extract-treated erythrocyte and unfractionated human plasma also showed lower TBARS and CD values. In addition, regeneration of glutathione was more obvious in grape seed extracts than in the rest of the tissues.
The underlying mechanism of these changes can be related mainly to increased antioxidant status. Cimin grape consumption may have beneficial effects on health maintenance.
分析 Cimin 葡萄的酚类成分，这种葡萄是土耳其受保护地理标志的 2 个葡萄品种之一，当地用于治疗一些疾病的症状，如阳痿和心血管疾病，并研究其在细胞内外环境模型中对氧化剂介质的抗氧化能力。
根据葡萄组织和使用的提取溶剂，将 Cimin 葡萄样品分为 5 组。从每种提取物中总酚和单体黄烷醇含量的测定中，推断出葡萄组织的自由基清除（DPPH）和抗脂质过氧化产物[硫代巴比妥酸反应物质（TBARS）和共轭二烯（CD）]水平。
种子的甲醇提取物具有最高的黄烷醇总量，其中儿茶素的含量为 4.034mM。种子提取物的 DPPH 活性比其他样品高 2 至 10 倍。用种子提取物处理的红细胞和未分级的人血浆也显示出较低的 TBARS 和 CD 值。此外，葡萄种子提取物中的谷胱甘肽再生比其他组织更为明显。
这些变化的潜在机制主要与抗氧化状态的增强有关。Cimin 葡萄的消费可能对维持健康有益。
