Busserolles Jérôme, Gueux Elyett, Balasińska Bozena, Piriou Yannick, Rock Edmond, Rayssiguier Yves, Mazur Andrzej
Centre de Recherche en Nutrition Humaine d'Auvergne, Unité des Maladies Métaboliques et Micronutriments, INRA, Theix, Saint-Genès-Champanelle, France.
Int J Vitam Nutr Res. 2006 Jan;76(1):22-7. doi: 10.1024/0300-9831.76.1.22.
In vitro evidence exists for the potential antioxidant benefits of procyanidin-rich extracts, but in vivo studies are scarce. We have evaluated the effects of selected procyanidin-rich extracts on oxidative stress in rats in condition of prolonged consumption of these compounds and also after single administration i.e. in postprandial conditions.
Rats were fed for 8 weeks with diets supplemented with either a grape seed extract (GE), a pine bark extract (PE), or a high-degree polymerized pine bark extract (HPE). An additional study was performed in order to assess the postprandial effect of these extracts on plasma antioxidant capacity. The ferric-reducing antioxidant power (FRAP) and thiobarbituric acid-reactive substances (TBARS) were determined in plasma. For lipid peroxidation study of heart tissue, homogenates were prepared and TBARS were measured after lipid peroxidation induced by FeSO4-ascorbate.
After 8 weeks of dietary treatment, total antioxidant capacity in plasma was significantly higher in the GE and PE groups as compared with the other two groups. Plasma TBARS concentrations and heart susceptibility to peroxidation were not significantly different between the groups. In the postprandial state, by comparing plasma antioxidant capacity 2 hours after ingestion of the different procyanidin-rich extracts (500 mg/kg body weight), we observed that FRAP values were higher in the procyanidin-rich extracts groups as compared with the control group. Moreover, plasma FRAP concentration was significantly higher in the GE group as compared with the other groups.
The results of the present experiment constitute positive evidence for an in vivo antioxidant effect at the plasma level of procyanidin-containing plant extracts.
富含原花青素的提取物具有潜在抗氧化益处的体外证据存在，但体内研究稀缺。我们评估了所选富含原花青素的提取物在大鼠长期摄入这些化合物的情况下以及单次给药后（即餐后状态）对氧化应激的影响。
用补充了葡萄籽提取物（GE）、松树皮提取物（PE）或高度聚合松树皮提取物（HPE）的饲料喂养大鼠8周。进行了另一项研究以评估这些提取物对血浆抗氧化能力的餐后效应。测定血浆中的铁还原抗氧化能力（FRAP）和硫代巴比妥酸反应性物质（TBARS）。对于心脏组织的脂质过氧化研究，制备匀浆并在硫酸亚铁 - 抗坏血酸诱导脂质过氧化后测量TBARS。
经过8周的饮食处理后，与其他两组相比，GE组和PE组血浆中的总抗氧化能力显著更高。各组之间血浆TBARS浓度和心脏对过氧化的敏感性无显著差异。在餐后状态下，通过比较摄入不同富含原花青素的提取物（500mg/kg体重）2小时后的血浆抗氧化能力，我们观察到富含原花青素的提取物组的FRAP值高于对照组。此外，与其他组相比，GE组的血浆FRAP浓度显著更高。
本实验结果为含原花青素的植物提取物在血浆水平的体内抗氧化作用提供了积极证据。
