Department of Microbiology, Shahid Beheshti University, Tehran, Iran.
Turk J Med Sci. 2014;44(3):511-4. doi: 10.3906/sag-1302-67.
BACKGROUND/AIM: To study the prevalence of blaVIM and blaIMP genes in metallo-β-lactamase (MBL)-producing burn isolates of Pseudomonas aeruginosa in relation with AmpC and extended-spectrum β-lactamase (ESBL) production.
Thirty-two carbapenem-resistant MBL-producing P aeruginosa burn isolates from Shahid Motahari Burn Hospital in Tehran were employed. Antibiotic susceptibility was determined to 13 antibiotics including imipenem and meropenem by disk diffusion. AmpC and ESBL production was detected by the AmpC disk test and combined disk diffusion assay, respectively, blaIMP and blaVIM gene carriage was shown by polymerase chain reaction and type-specific primers.
AmpC production was observed in 81% and ESBL production was detected in 12.5% of the isolates. blalMP carriage was observed in 56.25% and blaVIM gene in 46.8% of the isolates. Surprisingly, 43.5% of the isolates carried both blalMP and blaviM genes.
We think that this is the first report on the cocarriage of blalMP and blavIM in P aeruginosa. There was also a strong association between MBL gene carriage and AmpC β-lactamase production.
背景/目的：研究与 AmpC 和超广谱β-内酰胺酶（ESBL）产生相关的铜绿假单胞菌产金属β-内酰胺酶（MBL）烧伤分离株中 blaVIM 和 blaIMP 基因的流行情况。
我们采用了来自德黑兰沙希德·莫塔哈里烧伤医院的 32 株耐碳青霉烯类 MBL 产铜绿假单胞菌烧伤分离株。通过纸片扩散法测定了 13 种抗生素（包括亚胺培南和美罗培南）的药敏性。通过 AmpC 圆盘试验和联合圆盘扩散试验分别检测 AmpC 和 ESBL 的产生，通过聚合酶链反应和特异性引物显示 blaIMP 和 blaVIM 基因携带情况。
81%的分离株产生 AmpC，12.5%的分离株产生 ESBL。56.25%的分离株携带 blaIMP 基因，46.8%的分离株携带 blaVIM 基因。令人惊讶的是，43.5%的分离株同时携带 blaIMP 和 blaVIM 基因。
我们认为这是首次报道铜绿假单胞菌同时携带 blaIMP 和 blaVIM 基因。MBL 基因携带与 AmpC β-内酰胺酶产生之间也存在很强的关联。
