Borase Hemant P, Salunkhe Rahul B, Patil Chandrashekhar D, Suryawanshi Rahul K, Salunke Bipinchandra K, Wagh Nilesh D, Patil Satish V
School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
School of Environment Science, North Maharashtra University, Jalgaon, Maharashtra, India.
Biotechnol Appl Biochem. 2015 Nov-Dec;62(6):780-4. doi: 10.1002/bab.1341. Epub 2015 Apr 1.
In the present study, a rapid, low-cost, and ecofriendly method of stable silver nanoparticles (AgNPs) synthesis using leaves extract of Ficus carica (F. carica), a plant with diverse metabolic consortium, is reported for the first time. An absorption peak at 422 nm in UV-Vis spectroscopy, a spherical shape with an average size of 21 nm in transmission electron microscopy, and crystalline nature in X-ray powder diffraction studies were observed for the synthesized AgNPs. Fourier transform infrared analysis indicated that proteins of F. carica might have a vital role in AgNP synthesis and stabilization. AgNPs were found to inhibit urease, a key enzyme responsible for the survival and pathogenesis of the bacterium, Helicobacter pylori. Inhibition of urease by AgNPs was monitored spectrophotometrically by the evaluation of ammonia release. The urease inhibition potential of AgNPs can be explored in the treatment of H. pylori by preparing novel combinations of standard drugs with AgNPs- or AgNPs-encapsulated drug molecules.
在本研究中，首次报道了一种利用具有多种代谢成分的无花果树叶提取物，快速、低成本且环保地合成稳定银纳米颗粒（AgNPs）的方法。对于合成的AgNPs，在紫外可见光谱中观察到422nm处的吸收峰，在透射电子显微镜下呈平均尺寸为21nm的球形，并且在X射线粉末衍射研究中具有晶体性质。傅里叶变换红外分析表明，无花果的蛋白质可能在AgNP的合成和稳定中起关键作用。发现AgNPs可抑制脲酶，脲酶是幽门螺杆菌生存和致病的关键酶。通过评估氨释放，用分光光度法监测AgNPs对脲酶的抑制作用。通过制备标准药物与AgNPs或AgNPs包裹的药物分子的新型组合，可探索AgNPs在幽门螺杆菌治疗中的脲酶抑制潜力。
