Yeo JuDong, Shahidi Fereidoon
Department of Biochemistry, Memorial University of Newfoundland , St. John's, Newfoundland, Canada A1B 3X9.
J Agric Food Chem. 2015 Jan 21;63(2):379-81. doi: 10.1021/jf505632p. Epub 2015 Jan 9.
A new indicator, the ratio of insoluble bound phenolics (IBPs) to soluble phenolics (SPs), is suggested as an effective means to monitor changes in the antioxidant activity of lentils during germination. This indicator may be used to monitor other process-induced changes in antioxidant potential of food phenolics in other foods. The antioxidant activity of SPs, IBPs, and total value, the sum of both free and esterified phenolics, of germinated CDC Richlea lentil variety was evaluated for 4 days. Total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging ability were employed to record antioxidant activities. An incremental increase in IBPs was found in TPC, TFC, DPPH, and ABTS radical cation scavenging ability, whereas SPs showed a declining trend in TFC, DPPH, and ABTS, except TPC during 4 days of germination. The ratio of IBPs to SPs increased using most methods, and this may be possibly due to the changes of phenolic compound formation from soluble into insoluble bound form during germination process. The ratio can be used as a novel method for monitoring process-induced changes in the antioxidant activity of foods.
一种新的指标，即不溶性结合酚（IBP）与可溶性酚（SP）的比率，被认为是监测发芽过程中小扁豆抗氧化活性变化的有效手段。该指标可用于监测其他食品中酚类物质抗氧化潜力的其他加工诱导变化。对发芽4天的CDC Richlea小扁豆品种的可溶性酚、不溶性结合酚以及总酚（游离酚和酯化酚之和）的抗氧化活性进行了评估。采用总酚含量（TPC）、总黄酮含量（TFC）、2,2-二苯基-1-苦基肼（DPPH）自由基和2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子清除能力来记录抗氧化活性。在发芽的4天中，不溶性结合酚在总酚含量、总黄酮含量、DPPH和ABTS自由基阳离子清除能力方面呈递增趋势，而可溶性酚在总黄酮含量、DPPH和ABTS方面呈下降趋势，但总酚含量除外。使用大多数方法时，不溶性结合酚与可溶性酚的比率会增加，这可能是由于发芽过程中酚类化合物从可溶性形式转变为不溶性结合形式所致。该比率可作为监测食品抗氧化活性加工诱导变化的一种新方法。
