Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
Food Chem. 2020 Jun 15;315:126202. doi: 10.1016/j.foodchem.2020.126202. Epub 2020 Jan 13.
The identification and quantification of soluble- and insoluble-bound phenolics in lentil hulls were studied using HPLC-DAD-ESI-MS and their antioxidant potential determined using DPPH radical scavenging ability (DRSA), reducing power (RP), and hydroxyl radical scavenging ability (HRSA) assays to test their electron and hydrogen donating abilities. A number of soluble phenolics such as phenolic acids, flavonoids, and proanthocyanidins were found, which lead to the remarkable antioxidant potential as reflected in DRSA, RP, and HRSA. Meanwhile, insoluble-bound phenolics displayed a relatively lower number of peaks and contents than their corresponding soluble phenolics, leading to a lower antioxidant potential than that of soluble phenolics. Moreover, dihydrokaempferol dimer and carboxylated kaempferol diglucoside were identified for the first time in the insoluble-bound form in lentils. This study offers important data for the identification of phenolic compounds derived from lentils and their antioxidant potential.
采用 HPLC-DAD-ESI-MS 法对菜豆壳中可溶结合态和不可溶结合态酚类物质进行了鉴定和定量分析，并通过 DPPH 自由基清除能力（DRSA）、还原能力（RP）和羟基自由基清除能力（HRSA）测定法测定了其抗氧化潜力，以测试其电子和氢供体能力。发现了一些可溶酚类物质，如酚酸、类黄酮和原花青素，这导致了其具有显著的抗氧化潜力，体现在 DRSA、RP 和 HRSA 中。同时，不可溶结合态酚类物质的峰数和含量相对低于其相应的可溶酚类物质，导致其抗氧化潜力低于可溶酚类物质。此外，二氢山奈酚二聚体和羧基化山奈酚双葡萄糖苷首次在菜豆中以不可溶结合态的形式被鉴定出来。这项研究为菜豆中酚类化合物的鉴定及其抗氧化潜力提供了重要数据。
