Chang T K, Li C Y, Smithson W A
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.
Arch Pathol Lab Med. 1989 Dec;113(12):1343-8.
Fourteen commercially available monoclonal antibodies were used to examine, by the indirect immunoperoxidase technique, 20 formaldehyde solution-fixed, routinely processed bone marrow biopsy and surgical biopsy specimens from a group of small round cell tumors with bone marrow involvement, including 6 neuroblastomas, 4 rhabdomyosarcomas, 3 Ewing's sarcomas, 3 Burkitt's lymphomas, and 4 small-cell carcinomas of the lung. Eight of 14 monoclonal antibodies worked well on the routinely processed sections. Antidesmin (D33) (Dako Corp, Santa Barbara, Calif) and anti-muscle actin (HHF35) showed specific immunostaining in all 4 rhabdomyosarcomas included in this study. Anti-epithelial membrane antigen immunoreactivity was positive in all small-cell carcinomas of the lung. Anti-leukocyte common antigen (Dako) showed positive immunostaining in lymphomas and did not immunoreact with nonhematopoietic tumors. However, it was important to be aware that some lymphomas may not have been labeled with anti-leukocyte common antigen. All 6 neuroblastomas showed a positive immunoreactivity with anti-chromogranin A (LK2H10) and a weak reaction with antisynaptophysin (SY38). Panels of commercially available monoclonal antibodies, including anti-leukocyte common antigen, anti-epithelial membrane antigen, antidesmin (Dako), anti-muscle actin, and anti-chromogranin A, appear to be most useful in the differential diagnosis and staging of small round cell tumors on routinely processed biopsy specimens.
使用14种市售单克隆抗体，通过间接免疫过氧化物酶技术，检测了20份甲醛溶液固定、常规处理的骨髓活检和手术活检标本，这些标本来自一组伴有骨髓受累的小圆形细胞肿瘤，包括6例神经母细胞瘤、4例横纹肌肉瘤、3例尤因肉瘤、3例伯基特淋巴瘤和4例肺小细胞癌。14种单克隆抗体中有8种在常规处理的切片上效果良好。抗结蛋白（D33）（Dako公司，加利福尼亚州圣巴巴拉）和抗肌肉肌动蛋白（HHF35）在本研究纳入的所有4例横纹肌肉瘤中均显示出特异性免疫染色。抗上皮膜抗原免疫反应在所有肺小细胞癌中均呈阳性。抗白细胞共同抗原（Dako）在淋巴瘤中显示阳性免疫染色，与非造血肿瘤无免疫反应。然而，必须注意的是，一些淋巴瘤可能未被抗白细胞共同抗原标记。所有6例神经母细胞瘤均与抗嗜铬粒蛋白A（LK2H10）呈阳性免疫反应，与抗突触素（SY38）呈弱阳性反应。包括抗白细胞共同抗原、抗上皮膜抗原、抗结蛋白（Dako）、抗肌肉肌动蛋白和抗嗜铬粒蛋白A在内的市售单克隆抗体组合，似乎对常规处理的活检标本中小圆形细胞肿瘤的鉴别诊断和分期最为有用。
