Corwin D J, Gown A M
Department of Pathology, University of Washington, Seattle.
Arch Pathol Lab Med. 1989 Jun;113(6):645-52.
This review discusses several commonly used lineage-directed antibodies, including those to cytokeratins, vimentin, glial fibrillary acidic protein, neurofilaments, desmin, actin, leukocyte common antigen, and melanoma-specific antigen. Discussion is particularly directed to issues of antibody sensitivity and specificity, with several confusing and/or potentially beneficial examples of cross-lineage antigenic co-expression cited. The value of using limited panels composed of antibodies with complementary specificities is also emphasized.
本综述讨论了几种常用的谱系定向抗体，包括针对细胞角蛋白、波形蛋白、胶质纤维酸性蛋白、神经丝、结蛋白、肌动蛋白、白细胞共同抗原和黑色素瘤特异性抗原的抗体。讨论特别针对抗体敏感性和特异性问题，引用了几个跨谱系抗原共表达的令人困惑和/或可能有益的例子。还强调了使用由具有互补特异性的抗体组成的有限抗体组合的价值。
