一种新型GABAA受体α亚基的序列与表达

Sequence and expression of a novel GABAA receptor alpha subunit.

作者信息

Ymer S, Draguhn A, Köhler M, Schofield P R, Seeburg P H

机构信息

Laboratory of Molecular Neuroendocrinology, Center for Molecular Biology (ZMBH), Heidelberg, FRG.

出版信息

FEBS Lett. 1989 Nov 20;258(1):119-22. doi: 10.1016/0014-5793(89)81630-8.

DOI:10.1016/0014-5793(89)81630-8

PMID:2556293

Abstract

Cloned cDNA encoding the bovine alpha 4 subunit of the GABAA receptor has been isolated. The predicted 51 amino acid long mature protein contains an exceptionally long intracellular domain and shares 53-56% sequence similarity to the previously characterized alpha 1, alpha 2 and alpha 3 subunits. Co-expression of alpha 4 and beta 1 in Xenopus oocytes resulted in the formation of GABA-gated chloride channels with expected pharmacology, although no benzodiazepine potentiation was observed. Northern analysis indicates that a 4 kb alpha 4 mRNA is expressed in the calf cerebellum, cortex and hippocampus but is barely detectable in the rat brain.

摘要

已分离出编码牛γ-氨基丁酸A（GABAA）受体α4亚基的克隆互补DNA（cDNA）。预测的由51个氨基酸组成的成熟蛋白含有一个异常长的细胞内结构域，与先前鉴定的α1、α2和α3亚基具有53%-56%的序列相似性。α4和β1在非洲爪蟾卵母细胞中共表达，形成了具有预期药理学特性的γ-氨基丁酸门控氯离子通道，尽管未观察到苯二氮䓬增强作用。Northern分析表明，4kb的α4信使核糖核酸（mRNA）在小牛的小脑、皮质和海马体中表达，但在大鼠脑中几乎检测不到。