Bruin G J, Huisden R, Kraak J C, Poppe H
Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
J Chromatogr. 1989 Oct 20;480:339-49. doi: 10.1016/s0021-9673(01)84303-x.
The walls of fused-silica capillaries were chemically modified with small carbohydrate moieties in order to diminish the wall adsorption of proteins in capillary zone electrophoresis. A diol-type coating, prepared by bonding of gamma-glycidoxy propyltrimethoxysilane to the wall followed by acidic hydrolysis, shows for proteins a similar electrophoretic behaviour as various pH values to a polyethylene glycol (PEG) coating tested previously. Although good peak shapes were obtained for proteins in the pH range 3-5, the efficiency on the diol coating is worse than that on the PEG coating. At higher pH values the peaks are deformed and the efficiency is lost. A maltose coating appears to shield the silica surface well for proteins up to pH 7. The peak shapes of proteins are acceptable, but the efficiency of the maltose coating is smaller than that on the diol coating. The diol coating is stable in the indicated pH range. However, with the maltose coating good stability is obtained only on adding an antimicrobial agent to the buffers.
为了减少毛细管区带电泳中蛋白质在管壁上的吸附，对熔融石英毛细管的管壁进行了化学修饰，使其带有小的碳水化合物部分。通过将γ-缩水甘油氧基丙基三甲氧基硅烷键合到管壁上，随后进行酸水解制备的二醇型涂层，对于蛋白质而言，在不同pH值下呈现出与先前测试的聚乙二醇（PEG）涂层相似的电泳行为。尽管在pH值3 - 5范围内蛋白质获得了良好的峰形，但二醇涂层上的分离效率比PEG涂层上的要差。在较高pH值下，峰会变形且分离效率丧失。麦芽糖涂层在pH值达到7时似乎能很好地屏蔽二氧化硅表面对蛋白质的吸附。蛋白质的峰形是可以接受的，但麦芽糖涂层的分离效率低于二醇涂层。二醇涂层在指定的pH范围内是稳定的。然而，对于麦芽糖涂层，只有在向缓冲液中添加抗菌剂时才能获得良好的稳定性。
