一种用于轮状病毒的噬菌斑杂交测定法。
A plaque hybridization assay for rotaviruses.
作者信息
Poncet D, Cohen J
机构信息
Station de Virologie et d'Immunologie Moléculaires, INRA, Jouy-en-Josas, France.
出版信息
J Virol Methods. 1989 Oct;26(1):27-33. doi: 10.1016/0166-0934(89)90071-2.
PMID:2556423
Abstract
A plaque hybridization assay was adapted to rotavirus. In this test cDNA or oligonucleotide probes were used to discriminate between plaques originating from virus carrying genes of bovine and simian origin. Only mRNAs present in infected cells were detected as demonstrated by using oligonucleotides corresponding to both strands. This assay can be used to screen reassortants or mutants of rotaviruses.
摘要
一种噬菌斑杂交试验被应用于轮状病毒。在该试验中，使用cDNA或寡核苷酸探针来区分源自携带牛源和猿猴源基因的病毒的噬菌斑。如使用与两条链相对应的寡核苷酸所证明的，仅检测感染细胞中存在的mRNA。该试验可用于筛选轮状病毒的重配体或突变体。
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