Qian Y, Saif L J, Kapikian A Z, Kang S Y, Jiang B, Ishimaru Y, Yamashita Y, Oseto M, Green K Y
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Arch Virol. 1991;118(3-4):269-77. doi: 10.1007/BF01314037.
The genetic relationship between human and porcine Gp C rotaviruses and between Gp C and Gp A or B rotaviruses was examined by Northern blot hybridization. Cross-hybridization studies using radiolabeled ssRNA transcript probes demonstrated that the human and porcine Gp C rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. Neither the human nor the porcine Gp C probe hybridized strongly with gene segments from Gp A reference strains or a Gp B bovine rotavirus. These data indicate that genetically, porcine and human Gp C rotaviruses are closely related, whereas they are quite distinct from Gp A or B suggesting that porcine and human Gp C rotaviruses may have evolved from a common ancestral source.
通过Northern印迹杂交研究了人源和猪源Gp C轮状病毒之间以及Gp C与Gp A或B轮状病毒之间的遗传关系。使用放射性标记的单链RNA转录本探针进行的交叉杂交研究表明，人源和猪源Gp C轮状病毒在11个基因片段中的大多数片段上具有高度的核苷酸序列同源性；在基因7中观察到最大的序列差异。人源和猪源Gp C探针均未与Gp A参考毒株或Gp B牛轮状病毒的基因片段强烈杂交。这些数据表明，在遗传上，猪源和人源Gp C轮状病毒密切相关，而它们与Gp A或B有很大不同，这表明猪源和人源Gp C轮状病毒可能起源于共同的祖先。
