黄芩苷、黄芩素和汉黄芩素对分泌型IIA组磷脂酶A2的抑制作用。

Inhibitory effect of baicalin, baicalein and wogonin on secretory group IIA phospholipase A2.

作者信息

Ku Sae-Kwang, Lee Hyun Gyu, Bae Jong-Sup

机构信息

Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea.

Department of Microbiology and Immunology, Yonsei University College of Medicine, Seoul, Republic of Korea.

出版信息

Arch Pharm Res. 2015 Oct;38(10):1865-72. doi: 10.1007/s12272-014-0540-0. Epub 2015 Jan 7.

DOI:10.1007/s12272-014-0540-0

PMID:25564337

Abstract

It is well known that the expression level of secretory group IIA phospholipase A2 (sPLA2-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) up-regulates the expression of sPLA2-IIA in human umbilical vein endothelial cells (HUVECs). Here, three structurally related polyphenols found in the Chinese herb Huang Qui, namely baicalin, baicalein, and wogonin, were examined for its effects on the expression and activity of sPLA2-IIA in HUVECs and mouse. Prior treatment of cells or mouse with baicalin, baicalein, and wogonin inhibited LPS-induced expression and activity of sPLA2-IIA. And each compound suppressed the activation of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK) 1/2 by LPS. Therefore, these results suggest that baicalin, baicalein, and wogonin inhibited LPS mediated expression of sPLA2-IIA by suppression of cPLA2 and ERK 1/2.

摘要

众所周知，分泌性IIA组磷脂酶A2（sPLA2-IIA）在炎症性疾病中的表达水平会升高，并且脂多糖（LPS）可上调人脐静脉内皮细胞（HUVECs）中sPLA2-IIA的表达。在此，对中药黄芪中发现的三种结构相关的多酚类物质，即黄芩苷、黄芩素和汉黄芩素，检测了它们对HUVECs和小鼠中sPLA2-IIA的表达及活性的影响。用黄芩苷、黄芩素和汉黄芩素对细胞或小鼠进行预处理，可抑制LPS诱导的sPLA2-IIA的表达及活性。并且每种化合物均可抑制LPS对胞质磷脂酶A2（cPLA2）和细胞外信号调节激酶（ERK）1/2的激活。因此，这些结果表明黄芩苷、黄芩素和汉黄芩素通过抑制cPLA2和ERK 1/2来抑制LPS介导的sPLA2-IIA的表达。