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正常人T细胞中白细胞介素3基因诱导的调控

Regulation of interleukin 3 gene induction in normal human T cells.

作者信息

Guba S C, Stella G, Turka L A, June C H, Thompson C B, Emerson S G

机构信息

Howard Hughes Medical Institute, University of Michigan, Ann Arbor 48109.

出版信息

J Clin Invest. 1989 Dec;84(6):1701-6. doi: 10.1172/JCI114352.

DOI:10.1172/JCI114352
PMID:2556442
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304045/
Abstract

The regulation of IL-3 gene induction in human peripheral blood T cells was studied. IL-3 gene expression was inducible by crosslinking of the T cell receptor/CD3 complex using anti-CD3 MAb G19-4. Anti-CD3-induced IL-3 gene expression was found to be limited to the CD28+ T cell subset and could be augmented by costimulating T lymphocytes with antibodies directed against CD28. IL-3 expression could also be induced by costimulation of T cells with both phorbol ester and ionomycin, which are thought to mimic the intracellular effects of T cell receptor-antigen interaction. However, unlike other lymphokines such as IL-2 or granulocyte-macrophage colony-stimulating factor, IL-3 gene expression is not induced by stimulation of cells with phorbol myristate acetate and anti-CD28. We conclude that IL-3 gene regulation is under stringent control since IL-3 gene expression occurs only in the CD28+ subset of T cells, and since IL-3 induction obligately requires increased intracellular calcium.

摘要

研究了人外周血T细胞中IL-3基因诱导的调控。使用抗CD3单克隆抗体G19-4交联T细胞受体/CD3复合物可诱导IL-3基因表达。发现抗CD3诱导的IL-3基因表达仅限于CD28+ T细胞亚群，并且通过用针对CD28的抗体共刺激T淋巴细胞可增强该表达。用佛波酯和离子霉素共刺激T细胞也可诱导IL-3表达，它们被认为可模拟T细胞受体-抗原相互作用的细胞内效应。然而，与其他淋巴因子如IL-2或粒细胞-巨噬细胞集落刺激因子不同，用佛波醇肉豆蔻酸酯乙酸盐和抗CD28刺激细胞不会诱导IL-3基因表达。我们得出结论，IL-3基因调控受到严格控制，因为IL-3基因表达仅发生在T细胞的CD28+亚群中，并且因为IL-3诱导必然需要细胞内钙增加。

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