Jung Woo-Sun, Yang Il-Hyung, Lim Won Hee, Baek Seung-Hak, Kim Tae-Woo, Ahn Sug-Joon
Dental Research Institute and Department of Orthodontics, School of Dentistry, Seoul National University, Seoul, Korea (ROK).
Dental Research Institute and Department of Orthodontics, School of Dentistry, Seoul National University, Seoul, Korea (ROK)
Eur J Orthod. 2015 Dec;37(6):565-9. doi: 10.1093/ejo/cju090. Epub 2015 Jan 6.
To analyze in vivo mutans streptococci (MS) adhesion to self-ligating ceramic brackets [Clarity-SL (CSL) and Clippy-C (CC)] and the relationships between bacterial adhesion and oral hygiene indices.
Four central incisor brackets from the maxilla and mandible were collected from 40 patients (20 patients per each bracket type) at debonding immediately after plaque and gingival indices were measured. Adhesions of Streptococcus mutans, S. sobrinus, and total bacteria were quantitatively determined using real-time polymerase chain reaction after genomic DNA was extracted. Factorial analysis of variance was used to analyze bacterial adhesion to the brackets with respect to the bracket type and jaw position. Correlation coefficients were calculated to determine the relationships of bacterial adhesion to oral hygiene indices.
Adhesion of total bacteria and S. mutans to CSL was higher than that to CC (P < 0.001). Adhesion of total bacteria to the mandibular brackets was higher than that to the maxillary ones (P < 0.001), while adhesion of S. mutans to the maxillary brackets were higher than that in the mandibular ones (P < 0.001). In particular, the proportion of S. mutans to total bacteria in CSL was higher than CC (P < 0.05) in the maxillary anterior teeth (P < 0.001). There were no significant differences in adhesion of S. sobrinus between the brackets and jaw positions. Interestingly, no significant relationships were found between bacterial adhesions and oral hygiene indices.
Complex bracket configurations may significantly influence bacterial adhesion to orthodontic brackets. Further in vivo study using bracket raw materials will help to define the relationships between bacteria adhesion and enamel demineralization.
Because oral hygiene indices were not significantly correlated with adhesions of MS to self-ligating ceramic brackets, careful examinations around the brackets should be needed to prevent enamel demineralization, regardless of oral hygiene status.
分析变形链球菌（MS）在体内对自锁式陶瓷托槽[Clarity-SL（CSL）和Clippy-C（CC）]的黏附情况以及细菌黏附与口腔卫生指数之间的关系。
在测量菌斑和牙龈指数后，立即从40例患者（每种托槽类型20例患者）口中收集上颌和下颌的4个中切牙托槽，在托槽拆除时进行研究。提取基因组DNA后，使用实时聚合酶链反应定量测定变形链球菌、远缘链球菌和总细菌的黏附情况。采用析因方差分析来分析不同托槽类型和颌位对细菌黏附的影响。计算相关系数以确定细菌黏附与口腔卫生指数之间的关系。
总细菌和变形链球菌在CSL上的黏附高于在CC上的黏附（P<0.001）。总细菌在下颌托槽上的黏附高于上颌托槽（P<0.001），而变形链球菌在上颌托槽上的黏附高于下颌托槽（P<0.001）。特别是在上颌前牙中，CSL中变形链球菌占总细菌的比例高于CC（P<0.05）（P<0.001）。远缘链球菌在不同托槽类型和颌位之间的黏附无显著差异。有趣的是，未发现细菌黏附与口腔卫生指数之间存在显著关系。
复杂的托槽结构可能会显著影响细菌对正畸托槽的黏附。进一步使用托槽原材料进行体内研究将有助于明确细菌黏附与牙釉质脱矿之间的关系。
由于口腔卫生指数与MS对自锁式陶瓷托槽的黏附无显著相关性，因此无论口腔卫生状况如何，都需要仔细检查托槽周围以预防牙釉质脱矿。
