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两种托槽结扎类型对菌斑滞留的影响:实时聚合酶链反应的定量微生物分析。

Effects of 2 bracket and ligation types on plaque retention: a quantitative microbiologic analysis with real-time polymerase chain reaction.

机构信息

Department of Orthodontics, Faculty of Dentistry, Selçuk University, Konya, Turkey.

出版信息

Am J Orthod Dentofacial Orthop. 2013 Aug;144(2):260-7. doi: 10.1016/j.ajodo.2013.03.022.

DOI:10.1016/j.ajodo.2013.03.022
PMID:23910207
Abstract

INTRODUCTION

The aim of this study was to evaluate the effects of self-ligating brackets and conventional brackets ligated with stainless steel ligatures on dental plaque retention and microbial flora.

METHODS

Twenty boys (mean age, 14.2 ± 1.5 years) underwent bonding with self-ligating bracket systems and conventional standard edgewise bracket systems ligated with stainless steel ligatures with a split-mouth design. Clinical measurements, including plaque index, probing pocket depth, and bleeding on probing, were obtained before bonding, 1 week after bonding, and 3 months after bonding. Supragingival plaque samples were obtained at baseline and 3 months after bonding for the detection of bacteria. A quantitative analysis for Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, and Lactobacillus acidophilus was performed using real-time polymerase chain reaction. The Mann-Whitney U test and the Hotelling T(2) multivariate test were used for statistical comparisons of the groups.

RESULTS

The numbers of S mutans, S sobrinus, L casei, and L acidophilus were not statistically different between self-ligating brackets and conventional brackets ligated with stainless steel ligatures (P >0.05). The 2 archwire ligation techniques showed no statistically significant differences in plaque index, bleeding on probing, and probing pocket depth values of the bonded teeth (P >0.05). All clinical parameters and the numbers of all microorganisms showed statistically significant increases from baseline to 3 months after bonding in both groups (P <0.001).

CONCLUSIONS

Self-ligating brackets and conventional brackets ligated with stainless steel ligatures do not differ with regard to dental plaque retention.

摘要

简介

本研究旨在评估自锁托槽和传统结扎托槽(不锈钢结扎丝结扎)对牙菌斑滞留和微生物群落的影响。

方法

采用分侧设计,将 20 名男性(平均年龄 14.2±1.5 岁)分别粘接自锁托槽系统和传统标准方丝弓托槽系统(不锈钢结扎丝结扎)。在粘接前、粘接后 1 周和 3 个月分别进行临床检查,包括菌斑指数、探诊深度和探诊出血。在基线和粘接后 3 个月时采集龈上菌斑样本,用于检测细菌。采用实时聚合酶链反应对变形链球菌、远缘链球菌、干酪乳杆菌和嗜酸乳杆菌进行定量分析。采用曼-惠特尼 U 检验和 Hotelling T(2)多变量检验对组间进行统计学比较。

结果

自锁托槽和传统结扎托槽(不锈钢结扎丝结扎)组之间的 S mutans、S sobrinus、L casei 和 L acidophilus 数量无统计学差异(P>0.05)。两种弓丝结扎技术在粘接牙的菌斑指数、探诊出血和探诊深度值方面无统计学差异(P>0.05)。两组所有临床参数和所有微生物数量均从基线到粘接后 3 个月呈统计学显著增加(P<0.001)。

结论

自锁托槽和传统结扎托槽(不锈钢结扎丝结扎)在牙菌斑滞留方面无差异。

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