Talaat Roba M, Abdel-Aziz Amal M, El-Maadawy Eman A, Abdel-Bary Naser
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University , Egypt and.
Immunol Invest. 2015;44(3):265-78. doi: 10.3109/08820139.2014.989328. Epub 2015 Jan 7.
Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin's lymphoma (NHL), this pilot study was designed to investigate the impact of CD38 (184C/G; rs6449182) and IL-6 (-174 G/C; rs1800795) gene polymorphism on susceptibility of Egyptians to diffuse large B cell lymphoma (DLBCL); major types of NHL. To the best of our knowledge, this study is the first one that examines CD38 polymorphism in the NHL. Genotyping polymorphism is performed using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) for CD38 and Mutagenically separated PCR (MS-PCR) for IL-6 in 100 Egyptian NHL patients with DLBCL subtype and 119 normal controls. The serum level of IL-6 was measured using Enzyme-linked immunosorbent assay (ELISA). CD38 (184C/G) genotype is significantly increased in NHL patients (p < 0.01), while the GG genotype is significantly increased in controls (p < 0.05). Only two genotypes were found (GG and GC) in IL-6 (-174), no CC in our NHL patients and only one case in the controls. Insignificant change in IL-6 (-174 G/C) genotypes was recorded. Significantly increased serum IL-6 (p < 0.05) was positively correlated (r = 0.17; p < 0.05) with the disease. Taken together, our data stressed the importance of CD38 gene polymorphism in developing DLBCL. Our pilot study indicates that CD38 (184) CG genotype might play a role in DLBCL susceptibility in Egyptians. Additional prospective studies on larger population are needed to confirm our findings.
鉴于了解非霍奇金淋巴瘤（NHL）发病机制中涉及的基因变异的重要性，本初步研究旨在调查CD38（184C/G；rs6449182）和白细胞介素-6（IL-6，-174 G/C；rs1800795）基因多态性对埃及人患弥漫性大B细胞淋巴瘤（DLBCL，NHL的主要类型）易感性的影响。据我们所知，本研究是首个检测NHL中CD38多态性的研究。采用限制性片段长度多态性-聚合酶链反应（RFLP-PCR）对100例埃及DLBCL亚型NHL患者和119例正常对照进行CD38基因分型多态性检测，采用诱变分离PCR（MS-PCR）对IL-6进行检测。采用酶联免疫吸附测定（ELISA）法检测血清IL-6水平。NHL患者中CD38（184C/G）基因型显著增加（p<0.01），而GG基因型在对照组中显著增加（p<0.05）。在IL-6（-174）中仅发现两种基因型（GG和GC），我们的NHL患者中无CC基因型，对照组中仅1例。IL-6（-174 G/C）基因型无显著变化。血清IL-6显著升高（p<0.05）与疾病呈正相关（r=0.17；p<0.05）。综上所述，我们的数据强调了CD38基因多态性在DLBCL发生中的重要性。我们的初步研究表明，CD38（184）CG基因型可能在埃及人患DLBCL的易感性中起作用。需要对更多人群进行进一步的前瞻性研究以证实我们的发现。
