Huang Jing, Xiao Rui, Shi Suyujie, Li Qingshu, Li Ming, Xiao Ming, Wang Yalan, Yang Yaying, Li Wenwen, Tang Yi
Department of Pathology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.
Molecular Medicine Diagnostic and Testing Center, Chongqing Medical University, Chongqing, China.
Sci Rep. 2025 Mar 13;15(1):8681. doi: 10.1038/s41598-025-92817-9.
To elucidate the relationship between circulating cytokines and the prognosis of microsatellite-stable(MSS) colorectal cancer (CRC) patients, we examined the correlation between circulating cytokine levels and tumor immune infiltration microenvironment in this patient population. By conducting a preliminary analysis of the GEO database, we identified five core genes associated with colorectal cancer and further analyzed their impact on immune infiltration. We measured serum cytokine levels and validated the immune infiltration results through immunohistochemical staining of common inflammatory cell markers, including CD3, CD4, CD8, CD163, and FOXP3. Our findings indicate that serum cytokine levels significantly influence immune infiltration in colorectal cancer, particularly IL6 and IFNγ, which play crucial roles. Specifically, the infiltration of M2-type macrophages and CD8 + T cells is correlated with serum levels of IL6 and IFNγ. MSS CRC patients with elevated IL6 expression exhibit improved prognosis.
为了阐明循环细胞因子与微卫星稳定（MSS）结直肠癌（CRC）患者预后之间的关系，我们在该患者群体中研究了循环细胞因子水平与肿瘤免疫浸润微环境之间的相关性。通过对基因表达综合数据库（GEO数据库）进行初步分析，我们确定了五个与结直肠癌相关的核心基因，并进一步分析了它们对免疫浸润的影响。我们测量了血清细胞因子水平，并通过对包括CD3、CD4、CD8、CD163和FOXP3在内的常见炎症细胞标志物进行免疫组化染色来验证免疫浸润结果。我们的研究结果表明，血清细胞因子水平显著影响结直肠癌中的免疫浸润，尤其是白细胞介素6（IL6）和干扰素γ（IFNγ），它们发挥着关键作用。具体而言，M2型巨噬细胞和CD8 + T细胞的浸润与IL6和IFNγ的血清水平相关。IL6表达升高的MSS CRC患者预后较好。
