Stanco Deborah, Viganò Marco, Perucca Orfei Carlotta, Di Giancamillo Alessia, Peretti Giuseppe M, Lanfranchi Luciano, de Girolamo Laura
Orthopaedics Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy.
IRCCS Galeazzi Orthopaedic Institute, Via R Galeazzi 4, 20161 Milan, Italy.
Regen Med. 2015;10(6):729-43. doi: 10.2217/rme.14.92. Epub 2015 Jan 7.
Adipose-derived stem cells (ASCs) have been deeply characterized for their usefulness in musculoskeletal tissue regeneration; recently, other mesenchymal stem cell (MSC) sources have also been proposed. This study compares for the first time human tendon stem/progenitor cells isolated from hamstring tendons with human ASCs.
MATERIALS & METHODS: Human TSPCs and ASCs were isolated from hamstring tendon portions and adipose tissue of healthy donors undergoing ACL reconstruction or liposuction, respectively (n = 7). Clonogenic ability, immunophenotype and multi-differentiation potential were assessed and compared.
Both populations showed similar proliferation and clonogenic ability and expressed embryonic stem cell genes and MSC surface markers. Tendon stem/progenitor cells showed lower adipogenic and osteogenic ability, but after the chondrogenic differentiation, they produced more abundant glycosaminoglycans and expressed higher levels of aggrecan with regards to ASCs. The tenogenic induction with BMP-12 upregulated SCX and DCN gene expression in both populations.
Our results demonstrate that waste hamstring tendon fragments could represent a convenient MSC source for musculoskeletal regenerative medicine.
脂肪来源干细胞（ASCs）在肌肉骨骼组织再生中的应用已得到深入研究；最近，也有人提出了其他间充质干细胞（MSC）来源。本研究首次将从绳肌腱中分离出的人肌腱干/祖细胞与人类ASCs进行比较。
分别从接受ACL重建的健康供体的绳肌腱部分和接受抽脂的健康供体的脂肪组织中分离出人TSPCs和ASCs（n = 7）。评估并比较了两者的克隆形成能力、免疫表型和多分化潜能。
两种细胞群体均表现出相似的增殖和克隆形成能力，并表达胚胎干细胞基因和MSC表面标志物。肌腱干/祖细胞的成脂和成骨能力较低，但在软骨分化后，与ASCs相比，它们产生了更丰富的糖胺聚糖，并表达了更高水平的聚集蛋白聚糖。用BMP - 12进行成腱诱导上调了两种细胞群体中SCX和DCN基因的表达。
我们的结果表明，废弃的绳肌腱碎片可能是肌肉骨骼再生医学中一种便捷的MSC来源。
