Tang Daoqiang, Liu Charles Yj, Shen Danping, Fan Shuqiong, Su Xinying, Ye Peng, Gavine Paul R, Yin Xiaolu
Department of Pathology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Department of Translational Science, Asia and Emerging Market iMed, AstraZeneca R&D, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Onco Targets Ther. 2014 Dec 16;8:7-14. doi: 10.2147/OTT.S70922. eCollection 2015.
To investigate the significance of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor (HER)2, and HER3 expression on survival outcomes in Chinese gastric cancer patients.
Formalin-fixed, paraffin-embedded specimens from 121 patients who underwent gastrectomy at Shanghai Renji Hospital from 2007-2010 were retrospectively examined. Fluorescence in situ hybridization and immunohistochemistry techniques were used to identify gene amplification and protein overexpression. Correlations between the expression or amplification of HER family genes and clinicopathological parameters were then determined using statistical analysis.
EGFR protein overexpression, an increase in HER2 copy number and gene amplification, and HER3 protein overexpression were identified in 33.1%, 17.4%, and 62.0% of samples, respectively. Statistical analysis showed a significant association between EGFR expression and tumor invasion depth or tumor stage. HER2 was also shown to be significantly associated with the tumor grade. In addition, EGFR protein overexpression was found to be significantly associated with worse overall survival (P=0.03).
The HER family members showed a high expression in gastric cancer. EGFR protein expression was associated with overall survival.
探讨表皮生长因子受体（EGFR）、人表皮生长因子受体（HER）2和HER3表达对中国胃癌患者生存结局的意义。
回顾性研究2007年至2010年在上海仁济医院接受胃切除术的121例患者的福尔马林固定、石蜡包埋标本。采用荧光原位杂交和免疫组织化学技术鉴定基因扩增和蛋白过表达。然后使用统计分析确定HER家族基因表达或扩增与临床病理参数之间的相关性。
分别在33.1%、17.4%和62.0%的样本中鉴定出EGFR蛋白过表达、HER2拷贝数增加和基因扩增以及HER3蛋白过表达。统计分析显示EGFR表达与肿瘤浸润深度或肿瘤分期之间存在显著关联。HER2也显示与肿瘤分级显著相关。此外，发现EGFR蛋白过表达与较差的总生存期显著相关（P = 0.03）。
HER家族成员在胃癌中呈高表达。EGFR蛋白表达与总生存期相关。
