Smogorzewski M, Campese V M, Massry S G
Division of Nephrology, University of Southern California School of Medicine, Los Angeles.
Kidney Int. 1989 Sep;36(3):458-65. doi: 10.1038/ki.1989.217.
Abnormalities in the function of the central nervous system exist in chronic renal failure (CRF) and some of these derangements may be related to excess parathyroid hormone (PTH) which causes a rise in brain calcium. The latter may affect metabolism of neurotransmitters such as norepinephrine (NE) in brain synaptosomes. We measured NE content, uptake and release in brain synaptosomes of CRF rats and studied whether excess PTH affects these parameters. Synaptosomes from rats with 21 days of CRF compared to those from normal animals have higher calcium content (11.4 +/- 0.92 vs. 7.1 +/- 0.50 nmol/mg protein, P less than 0.01) and lower Na-K ATPase activity (6.5 +/- 0.81 vs. 11.4 +/- 0.76 mumol Pi/mg protein/hr, P less than 0.01). NE content (11.0 +/- 0.60 vs. 13.6 +/- 0.55 pmol/mg protein/hr, P less than 0.01), uptake (46 +/- 4.5 vs. 110 +/- 5.9 pmol/mg protein times 50 min, P less than 0.01) and release (2.0 +/- 0.2 vs. 5.1 +/- 0.47 pmol/mg protein times 10 min, P less than 0.01). Parathyroidectomy (PTX) in CRF rats kept normocalcemic reversed these abnormalities in brain synaptosomes; indeed calcium content, Na-K ATPase activity and NE content, uptake and release in synaptosomes from PTX-CRF rats were not different from those seen in normal rats.(ABSTRACT TRUNCATED AT 400 WORDS)
慢性肾衰竭（CRF）患者存在中枢神经系统功能异常，其中一些紊乱可能与甲状旁腺激素（PTH）过多有关，PTH会导致脑钙含量升高。后者可能会影响脑突触体中去甲肾上腺素（NE）等神经递质的代谢。我们测量了CRF大鼠脑突触体中的NE含量、摄取和释放，并研究了过量的PTH是否会影响这些参数。与正常动物相比，CRF 21天的大鼠的突触体具有更高的钙含量（11.4±0.92对7.1±0.50 nmol/mg蛋白质，P<0.01）和更低的钠钾ATP酶活性（6.5±0.81对11.4±0.76 μmol Pi/mg蛋白质/小时，P<0.01）。NE含量（11.0±0.60对13.6±0.55 pmol/mg蛋白质/小时，P<0.01）、摄取（46±4.5对110±5.9 pmol/mg蛋白质×50分钟，P<0.01）和释放（2.0±0.2对5.1±0.47 pmol/mg蛋白质×10分钟，P<0.01）。CRF大鼠进行甲状旁腺切除术（PTX）并维持血钙正常可逆转脑突触体中的这些异常；实际上，PTX-CRF大鼠突触体中的钙含量、钠钾ATP酶活性以及NE含量、摄取和释放与正常大鼠无异。（摘要截取自400字）
