Wang Youhua, Zhou Shiran, Xu Hua, Yan Shixian, Xu Dawei, Zhang Yi
Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
J Mol Neurosci. 2015 May;56(1):216-27. doi: 10.1007/s12031-014-0484-3. Epub 2015 Jan 8.
Nuclear factor (NF)45 (also known as interleukin enhancer-binding factor (ILF)2), is a transcription factor that interacts with NF90 to regulate gene expression. It has long been implicated in the regulation of cell proliferation. However, the role of NF45 in the process of peripheral nervous system regeneration after injury remains poorly understood. Herein, we investigated the spatiotemporal expression of NF45 in a rat sciatic nerve crush model. We detected the up-regulated expression of NF45 in Schwann cell after sciatic nerve crush. What's more, the expression of the cell proliferation marker proliferating cell nuclear antigen (PCNA) exhibited a similar tendency with that of NF45. In cell cultures, we observed increased expression of NF45 during the process of TNF-α-induced Schwann cell proliferation, whereas the protein level of p21 was down-regulated. Interference of NF45 led to enhanced expression of p21 and also impaired proliferation of Schwan cells. Taken together, our data implicated that NF45 was up-regulated in the sciatic nerve after crush, which was associated with proliferation of Schwann cell.
核因子（NF）45（也称为白细胞介素增强子结合因子（ILF）2）是一种与NF90相互作用以调节基因表达的转录因子。长期以来，它一直被认为与细胞增殖的调节有关。然而，NF45在损伤后周围神经系统再生过程中的作用仍知之甚少。在此，我们在大鼠坐骨神经挤压模型中研究了NF45的时空表达。我们检测到坐骨神经挤压后雪旺细胞中NF45表达上调。此外，细胞增殖标志物增殖细胞核抗原（PCNA）的表达与NF45的表达呈现相似趋势。在细胞培养中，我们观察到在TNF-α诱导雪旺细胞增殖过程中NF45表达增加，而p21的蛋白水平下调。干扰NF45导致p21表达增强，同时也损害了雪旺细胞的增殖。综上所述，我们的数据表明挤压后坐骨神经中NF45上调，这与雪旺细胞的增殖有关。
