Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
Cell Mol Neurobiol. 2013 Jul;33(5):689-98. doi: 10.1007/s10571-013-9935-6. Epub 2013 Apr 9.
As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (SNC) model in adult rats to examine the dynamic changes of PHB2. Temporally, PHB2 expression was sharply decreased after sciatic nerve crush and reached a valley at day 5. Spatially, PHB2 was widely expressed in the normal sciatic nerve including axons and Schwann cells. While after injury, PHB2 expression decreased predominantly in Schwann cells. The alteration was due to the decreased expression of PHB2 in Schwann cells after SNC. PHB2 expression correlated closely with Schwann cells proliferation in sciatic nerve post injury. Furthermore, PHB2 largely localized with GAP43 in axons in the crushed segment. Collectively, we suggested that PHB2 participated in the pathological process response to sciatic nerve injury and may be associated with Schwann cells proliferation and axons regeneration.
作为一种新型的细胞周期抑制剂，PHB2 以复杂的方式控制细胞周期中 G1/S 期的转换。其异常表达与细胞癌变密切相关。然而，其在外周神经系统损伤和修复中的表达和作用尚不清楚。在这里，我们在成年大鼠中建立了急性坐骨神经挤压（SNC）模型，以研究 PHB2 的动态变化。在时间上，坐骨神经挤压后 PHB2 的表达急剧下降，在第 5 天达到谷值。在空间上，PHB2 在正常坐骨神经中广泛表达，包括轴突和施万细胞。然而，损伤后，PHB2 主要在施万细胞中表达减少。这种变化是由于 SNC 后施万细胞中 PHB2 的表达减少所致。PHB2 的表达与损伤后坐骨神经中施万细胞的增殖密切相关。此外，PHB2 在挤压段的轴突中与 GAP43 大量共存。综上所述，我们认为 PHB2 参与了坐骨神经损伤的病理过程反应，可能与施万细胞增殖和轴突再生有关。
