Cheng Hongxia, Ye Lun, Xue Liquan
Department of Chemical and Pharmaceutical Engineering, Wuhan University of Technology Huaxia College, Wuhan 430223, China.
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Zhonghua Zhong Liu Za Zhi. 2014 Oct;36(10):751-4.
To detect the frequency of ROS1 gene rearrangement in non-small cell lung cancer ( NSCLC) patients by FISH, and to analyze the relationship between ROS1 gene rearrangement and clinical features (including age, sex, stage, histology, smoking history) with NSCLC.
The ROS1 gene rearrangement in histological sections of 1 652 NSCLC tissues was detected by FISH. The extracted RNA was amplified and the sequences were analyzed by Sanger sequencing for ROS1-positive samples.
ROS1 rearrangement was identified in 53 specimens (3.2%) from the 1 652 NSCLC tissues. Among these positive cases, 15 were CD74-ROS1, 13 were SLC34A2-ROS1, 13 were SDC4-ROS1 and 12 were TPM3-ROS1. The frequency of ROS1 rearrangement was significantly higher in never-smoking patients (49 cases) than in smokers (4 cases) (P < 0.05). Patients with ROS1-positive NSCLC tended to be younger and there was no significant difference in sex (P > 0.05). All of the ROS1-positive samples were adenocarcinomas, with a tendency toward higher clinical stage (P < 0.05).
ROS1 rearrangement has diversity, and may be defined as a new molecular subtype of NSCLC. ROS1 rearrangement tends to occur in younger, and never-smoker lung adenocarcinoma patients.
采用荧光原位杂交（FISH）技术检测非小细胞肺癌（NSCLC）患者中ROS1基因重排的频率，并分析ROS1基因重排与NSCLC临床特征（包括年龄、性别、分期、组织学类型、吸烟史）之间的关系。
采用FISH技术检测1652例NSCLC组织病理切片中的ROS1基因重排。对提取的RNA进行扩增，并对ROS1阳性样本进行Sanger测序分析。
在1652例NSCLC组织中，53例（3.2%）检测到ROS1重排。在这些阳性病例中，15例为CD74-ROS1，13例为SLC34A2-ROS1，13例为SDC4-ROS1，12例为TPM3-ROS1。从不吸烟者（49例）中ROS1重排的频率显著高于吸烟者（4例）（P<0.05）。ROS1阳性的NSCLC患者倾向于更年轻，性别上无显著差异（P>0.05）。所有ROS1阳性样本均为腺癌，且倾向于更高的临床分期（P<0.05）。
ROS1重排具有多样性，可能被定义为NSCLC的一种新分子亚型。ROS1重排倾向于发生在年轻、从不吸烟的肺腺癌患者中。
