Huang Yunxin, Lloyd Alun L, Legros Mathieu, Gould Fred
Department of Entomology, North Carolina State University Raleigh, NC, USA.
Department of Mathematics, North Carolina State University Raleigh, NC, USA.
Evol Appl. 2011 May;4(3):415-28. doi: 10.1111/j.1752-4571.2010.00153.x. Epub 2010 Sep 14.
The potential benefits and risks of genetically engineered gene-drive systems for replacing wild pest strains with more benign strains must be assessed prior to any field releases. We develop a computer simulation model to assess the feasibility of using engineered underdominance constructs to drive transgenes into age- and spatially structured mosquito populations. Our practical criterion for success is the achievement of a transgene frequency of at least 0.80 within 3 years of release. The impacts of a number of parameters that may affect the success of gene-drive, such as the release area, release age, density-dependent larval survival, fitness cost of the engineered genes, and migration probability of adults, are examined. Results show that patchy release generally requires the release of fewer engineered insects to achieve success than central release. When the fitness cost is very low, central release covering 25% of the total area can be more effective than a completely uniform release over the whole area. This study demonstrates that to determine the best method of spatial release, and the total number of engineered insects that must be released, it is important to take into account the age and sex of the released insects and spatial structure of the population.
在进行任何野外释放之前，必须评估利用基因工程基因驱动系统用更良性的品系取代野生害虫品系的潜在益处和风险。我们开发了一个计算机模拟模型，以评估使用工程化的隐性致死构建体将转基因导入具有年龄和空间结构的蚊子种群的可行性。我们成功的实际标准是在释放后的3年内实现转基因频率至少达到0.80。研究了一些可能影响基因驱动成功的参数的影响，如释放区域、释放年龄、密度依赖的幼虫存活率、工程基因的适合度代价以及成虫的迁移概率。结果表明，与集中释放相比，分散释放通常需要释放更少的工程昆虫来取得成功。当适合度代价非常低时，覆盖总面积25%的集中释放可能比在整个区域完全均匀释放更有效。这项研究表明，要确定空间释放的最佳方法以及必须释放的工程昆虫总数，考虑释放昆虫的年龄和性别以及种群的空间结构非常重要。
