Bono M R, Benech P, Couillin P, Alcaide-Loridan C, Grisard M C, Jouin H, Fischer D G, Fellous M
Institut Pasteur-INSERM U.276.
Somat Cell Mol Genet. 1989 Nov;15(6):513-23. doi: 10.1007/BF01534912.
A panel of 27 rodent-human somatic cell hybrids composed of cells of hematopoietic (nonadherent cells) and nonhematopoietic origin (adherent cells) was used to identify the chromosomes involved in the biological response to human IFN-gamma (Hu-IFN-gamma). We found that the stimulation of class-I histocompatibility antigen expression correlates with the presence of human chromosomes 6 and 21 in adherent cell hybrids, while human chromosome 6 alone is sufficient in nonadherent hybrids. Scatchard analysis of the binding of radiolabeled Hu-IFN-gamma to nonadherent cell hybrids gave a Kd value similar to that found on human cell lines. Induction of a reporter gene placed under the transcriptional control of the interferon responsive sequence (IRS) in adherent cell hybrids requires both chromosomes 6 and 21. The antiviral protection by Hu-IFN-gamma in adherent cell hybrids was reached at physiological doses (2 units/ml) when human chromosomes 6 and 21 were present, while higher doses of Hu-IFN-gamma (5000 units/ml) were required for hybrids lacking chromosome 21. Thus, we demonstrate that differences exit in the response to Hu-IFN-gamma depending on the origin of the cell type.
使用一组由造血细胞（非贴壁细胞）和非造血细胞（贴壁细胞）组成的27种啮齿动物-人类体细胞杂种来鉴定参与对人γ干扰素（Hu-IFN-γ）生物学反应的染色体。我们发现，I类组织相容性抗原表达的刺激与贴壁细胞杂种中人染色体6和21的存在相关，而在非贴壁杂种中单独的人染色体6就足够了。对放射性标记的Hu-IFN-γ与非贴壁细胞杂种结合的Scatchard分析得出的Kd值与在人细胞系上发现的值相似。在贴壁细胞杂种中，置于干扰素反应序列（IRS）转录控制下的报告基因的诱导需要染色体6和21。当存在人染色体6和21时，Hu-IFN-γ在贴壁细胞杂种中的抗病毒保护在生理剂量（2单位/毫升）时即可实现，而对于缺乏染色体21的杂种则需要更高剂量的Hu-IFN-γ（5000单位/毫升）。因此，我们证明，根据细胞类型的来源，对Hu-IFN-γ的反应存在差异。
