Bono M R, Alcaïde-Loridan C, Letouzé B, Jouin H, Gobin S J, Fellous M
Institut National de la Santé et de la Recherche Médicale U.276, Paris, France.
Res Immunol. 1991 Nov-Dec;142(9):765-72. doi: 10.1016/0923-2494(91)90122-y.
It has previously been shown that stimulation of class I MHC antigen expression by human interferon gamma (Hu-IFN gamma) depends on the presence of both human chromosomes 6 and 21. Even although the presence of human chromosome 6 results in normal expression of the IFN gamma receptor on the cell surface, chromosome 21 is required for this biological response. Here, we transfected the cDNA encoding the Hu-IFN gamma receptor into a mouse cell line, L(tk-), and into a mouse x human hybrid, GM08, containing the human chromosome 21 as the only human genetic material. Two clones, L-gamma R.8 and GM08-gamma R.9, were tested for different biological responses to Hu-IFN gamma. Only the hybrid containing the human chromosome 21 (GM08-gamma R.9) exhibited a biological response when assayed for the stimulation of murine class I MHC antigen expression by Hu-IFN gamma. Moreover, transcriptional induction of 2'-5'-oligoadenylate synthetase and 1-8 genes, as well as antiviral protection, were observed only in the GM08-gamma R.9 transfectants. These results show that human chromosome 21 encodes a factor which mediates different biological activities of Hu-IFN gamma.
先前的研究表明，人干扰素γ（Hu-IFNγ）对I类MHC抗原表达的刺激作用依赖于人类6号和21号染色体的同时存在。尽管人类6号染色体的存在会导致IFNγ受体在细胞表面正常表达，但这种生物学反应还需要21号染色体。在此，我们将编码Hu-IFNγ受体的cDNA转染到小鼠细胞系L(tk-)以及仅含人类21号染色体作为唯一人类遗传物质的小鼠-人杂交细胞GM08中。对两个克隆L-γR.8和GM08-γR.9进行了对Hu-IFNγ不同生物学反应的测试。当检测Hu-IFNγ对小鼠I类MHC抗原表达的刺激作用时，只有含人类21号染色体的杂交细胞（GM08-γR.9）表现出生物学反应。此外，仅在GM08-γR.9转染细胞中观察到2'-5'-寡腺苷酸合成酶和1-8基因的转录诱导以及抗病毒保护作用。这些结果表明，人类21号染色体编码一种介导Hu-IFNγ不同生物学活性的因子。
