Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba, Japan (Drs Yang, Shirayama, Zhang, Ren, and Hashimoto); Department of Psychiatry, Teikyo University Chiba Medical Center, Ichihara, Japan (Dr Shirayama).
Int J Neuropsychopharmacol. 2015 Jan 7;18(7):pyu121. doi: 10.1093/ijnp/pyu121.
In the learned helplessness (LH) paradigm, approximately 35% of rats are resilient to inescapable stress.
The roles of brain-derived neurotrophic factor (BDNF) and dendritic spine density in the brain regions of LH (susceptible) and non-LH rats (resilient) were examined. Western blot analysis and Golgi staining were performed.
BDNF levels in the medial prefrontal cortex, CA3, and dentate gyrus (DG) were significantly lower in the LH group than in the control and non-LH groups, whereas BDNF levels in the nucleus accumbens (NAc) in the LH group but not the non-LH group were significantly higher than those in the control group. Furthermore, spine density in the prelimbic cortex, CA3, and DG was significantly lower in the LH group than in the control and non-LH groups, although spine density in the NAc was significantly higher in the LH group than in the control and non-LH groups.
The results suggest that regional differences in BDNF levels and spine density in rat brain may contribute to resilience to inescapable stress.
在习得性无助（LH）范式中，大约 35%的大鼠对不可逃避的压力具有弹性。
研究了大脑源性神经营养因子（BDNF）和树突棘密度在 LH（易感）和非 LH 大鼠（弹性）脑区中的作用。进行了 Western blot 分析和高尔基染色。
LH 组大鼠前额皮质、CA3 和齿状回（DG）的 BDNF 水平明显低于对照组和非 LH 组，而 LH 组伏隔核（NAc）的 BDNF 水平明显高于对照组，但非 LH 组则低于对照组。此外，LH 组大鼠前额皮质、CA3 和 DG 的棘突密度明显低于对照组和非 LH 组，而 LH 组大鼠 NAc 的棘突密度明显高于对照组和非 LH 组。
这些结果表明，大鼠大脑中 BDNF 水平和棘突密度的区域差异可能有助于对不可逃避的压力产生弹性。
