Abdallah Marwan, Benoliel Corinne, Ferreira-Theret Pauline, Drider Djamel, Dhulster Pascal, Chihib Nour-Eddine
a Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), IUT A/Polytech'Lille , Université de Lille1-Science et Technologies , Villeneuve-d'Ascq , France.
Biofouling. 2015;31(1):49-59. doi: 10.1080/08927014.2014.993390.
The relationship between the environmental conditions of biofilm formation and resistance to disinfectants was studied. Anti-biofilm assays were performed against biofilms grown at 20, 30 and 37°C on stainless steel and polycarbonate, over 24 and 48 h. A rise in growth temperature increased the resistance of 24 h biofilms to disinfectants containing didecyldimethylammonium chloride and decreased it to a disinfectant containing alkyldimethylbenzylammonium chloride. The increase in growth temperature coupled with an incubation time of 24 h promoted increases in both matrix production and the membrane rigidity of sessile cells. An increase in incubation time also increased both matrix production and the membrane rigidity of sessile cells. Such phenomena resulted in an increased resistance to disinfectants of biofilms grown at 20 and 30°C. The resistance of 48 h biofilms to disinfectants decreased with an increase in growth temperature despite the increase in matrix production and the membrane rigidity of sessile cells.
研究了生物膜形成的环境条件与对消毒剂抗性之间的关系。针对在20、30和37°C下于不锈钢和聚碳酸酯上生长24小时和48小时的生物膜进行了抗生物膜试验。生长温度的升高增加了24小时生物膜对含双癸基二甲基氯化铵的消毒剂的抗性，并降低了其对含烷基二甲基苄基氯化铵的消毒剂的抗性。生长温度的升高加上24小时的孵育时间促进了基质产生和固着细胞的膜刚性的增加。孵育时间的增加也增加了基质产生和固着细胞的膜刚性。这些现象导致在20和30°C下生长的生物膜对消毒剂的抗性增加。尽管固着细胞的基质产生和膜刚性增加，但48小时生物膜对消毒剂的抗性随着生长温度的升高而降低。
