He Dan-Dan, Liu Wu, Sun Ru, Fan Chen, Xu Yu-Jie, Ge Jian-Feng
College of Chemistry, Chemical Engineering and Material Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, ‡School of Radiation Medicine and Protection, Medicinal College, Soochow University , 199 Ren'Ai Road, Suzhou 215123, People's Republic of China.
Anal Chem. 2015 Feb 3;87(3):1499-502. doi: 10.1021/ac5045912. Epub 2015 Jan 12.
The lysosome-targetable OFF-ON type pH sensor that does not emit at pH = 4.0 is adopted for the selective detection of cancer cells, and the acidity difference of lysosomes in cancer and normal cells is verified. Three pH probes based on Darrow Red derivatives were designed and prepared that were demonstrated to be lysosome-specific biomarkers with inducible emission at 580-850 nm by the comparable in cellular imaging assays using HeLa, KB, and V79 cells. Of these, a pyridineium-2-yl Darrow Red analogue with a pKa of 2.4 was found to be a lysosome tracker for cancer cells, it is a unique pH sensor for the optical identification and distinction of cancer cells from normal cells and has potential application as a fluorescent biomaker of cancer cells in in vitro assays.
采用在pH = 4.0时不发光的可靶向溶酶体的开-关型pH传感器来选择性检测癌细胞，并验证了癌细胞和正常细胞中溶酶体的酸度差异。设计并制备了三种基于达罗红衍生物的pH探针，通过使用HeLa、KB和V79细胞的细胞成像分析表明，这些探针是溶酶体特异性生物标志物，在580 - 850 nm处有诱导发射。其中，pKa为2.4的吡啶-2-基达罗红类似物被发现是癌细胞的溶酶体追踪剂，它是一种独特的pH传感器，用于从正常细胞中光学识别和区分癌细胞，并且在体外分析中作为癌细胞的荧光生物标志物具有潜在应用。
