[几种阿米巴病免疫诊断方法的结果与经验]

[Results and experiences with several methods for the immunodiagnosis of amebiasis].

作者信息

Sauer H, Ockert G, Schubert S, Rothenberg G, Stein H E, Teichmann A

机构信息

Klinische Parasitologie des Medizinisch-Poliklinischen Institutes des Bereiches Medizin der Karl-Marx-Universität Leipzig, DDR.

出版信息

Angew Parasitol. 1989 Nov;30(4):209-13.

PMID:2556950

Abstract

Results and experiences of some immunodiagnostic methods for amebiasis. Experiences concerning the laboratory diagnosis of invasive amebiasis are described and discussed. Technics used include the Passive Hemagglutination Test with E. histolytica antigen (Behring FRG) and sheep erythrocytes as carrier and the Indirect Fluorescence Antibody Reaction (IFAR) with corpuscular antigen prepared from cultures of E. histolytica strain HK 9. Comparatively for a part infected hamster liver (Dr. Richle strain) has been used as antigen. We conclude that IFAR with HK 9 antigen is more sensitive than the other tests. It has been proved useful in the diagnosis of extraintestinal amebiasis (antibody titer greater than or equal to 1:160).

摘要

一些阿米巴病免疫诊断方法的结果与经验。描述并讨论了有关侵袭性阿米巴病实验室诊断的经验。所使用的技术包括以溶组织内阿米巴抗原（德国贝林公司）和绵羊红细胞为载体的被动血凝试验，以及用溶组织内阿米巴HK 9菌株培养物制备的颗粒抗原进行的间接荧光抗体反应（IFAR）。相对而言，部分感染的仓鼠肝脏（里克尔博士菌株）已被用作抗原。我们得出结论，用HK 9抗原的IFAR比其他试验更敏感。它已被证明在肠外阿米巴病的诊断中有用（抗体滴度大于或等于1:160）。