University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia.
University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia; University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Sci Total Environ. 2015 Apr 1;511:393-8. doi: 10.1016/j.scitotenv.2014.12.072. Epub 2015 Jan 5.
New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.
新德里金属β-内酰胺酶 1（NDM-1）由于其快速的国际传播以及能够被许多革兰氏阴性病原体表达，很快将成为世界上最常见的分离和分布的金属β-内酰胺酶。NDM 阳性细菌在印度次大陆和巴尔干地区构成了重大的公共卫生威胁，这些地区已被定为地方性区域。我们的研究集中在城市河流、湖泊和泉水上，这些可能是塞尔维亚产 NDM-1 菌株的潜在来源，也是其他金属β-内酰胺酶和扩展谱β-内酰胺酶（ESBL）产生菌的潜在来源。总共从贝尔格莱德的 10 个不同地点中的 8 个收集了 69 个β-内酰胺耐药分离株，属于 12 个细菌属，其中大多数来自一个受欢迎的娱乐场所 Ada Ciganlija 湖。表型测试显示 7（10.14%）个 ESBL 产生分离株和 39（56.52%）个对亚胺培南耐药的分离株，其中 32 个对金属β-内酰胺酶（MBL）产生呈阳性。PCR 和测序显示存在 SHV（3 个分离株）、DHA-1（1 个分离株）和 CMY-2（1 个分离株）β-内酰胺酶的遗传决定因素。然而，我们没有检测到任何产 NDM-1 的菌株（以前描述的塞尔维亚产 NDM-1 病例仅限于贝尔格莱德），因此我们提出塞尔维亚的 NDM-1 实际上是一种移植和医院获得性问题，而不是环境问题，塞尔维亚也不是 NDM-1 的地方性区域。
