纳米光栅结构促进基于片状伪足的细胞集体迁移和伤口愈合。
Nanograting structure promotes lamellipodia-based cell collective migration and wound healing.
作者信息
Chen Zaozao, Atchison Leigh, Ji HaYeun, Leong Kam W
出版信息
Annu Int Conf IEEE Eng Med Biol Soc. 2014;2014:2916-9. doi: 10.1109/EMBC.2014.6944233.
Abstract
Wound healing is a dynamic and complex process of replacing missing or dead cell structures and tissue layers. The aim of this research is to discover biocompatible materials and drugs that can promote cell migration in the wound area and thus enhance desirable wound healing effects. In this paper, we report that PDMS nanogratings could accelerate the migration of epithelial cells along the grating axis, and the addition of Imatinib could further increase the epithelial cell wound healing speed to 1.6 times the speed of control cells. We also demonstrate that this migration is mediated by lamellipodia protrusion, and is Rac1-GTPase activity dependent. Lastly, we discuss the potential application and prospect of different nanostructured biomaterials for wound healing studies.
摘要
伤口愈合是一个动态且复杂的过程，用于替换缺失或死亡的细胞结构及组织层。本研究的目的是发现能够促进伤口区域细胞迁移从而增强理想伤口愈合效果的生物相容性材料和药物。在本文中，我们报告聚二甲基硅氧烷（PDMS）纳米光栅可加速上皮细胞沿光栅轴的迁移，并且添加伊马替尼可进一步将上皮细胞伤口愈合速度提高至对照细胞速度的1.6倍。我们还证明这种迁移是由片状伪足突出介导的，并且依赖于Rac1 - GTP酶活性。最后，我们讨论了不同纳米结构生物材料在伤口愈合研究中的潜在应用和前景。
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