Wang Xikai, Zhang Yanqiu, Fu Yanyun, Zhang Juan, Yin Lihong, Pu Yuepu, Liang Geyu
Key Laboratory of Environmental Medicine Engineering Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
Mol Med Rep. 2015 May;11(5):3880-7. doi: 10.3892/mmr.2014.3142. Epub 2014 Dec 31.
The present study aimed to investigate the biofunctions of microRNA (miR)‑125b on lung cancer cells. A miR genechip array was used to examine the differential expression of miRs between 95D lung cancer cells and 16 human bronchial epithelial (HBE) cells. Overexpression of miR‑125b was observed in the cell lines and in the lung carcinoma tissues compared with the adjacent tissues, confirmed using reverse transcription quantitative polymerase chain reaction. Bioinformatic analysis of miR‑125b was also performed, including target prediction, gene ontology and pathway analysis. MTT, flow cytometry and Transwell assays were also used to examine the effect of downregulated miR‑125b on the proliferation, apoptosis, invasive ability and cell cycle of 95D cells. Significant differences were observed in the expression of 45 miRs in the 95D cells compared with those in 16HBE cells and the expression of miR‑125b was significantly higher in 95D cells compared with that in 16HBE cells as well as in lung tumor tissues compared with that in adjacent tissues. In addition, inhibition of the expression of miR‑125b in 95D cells induced apoptosis, G1/S phase arrest and reduction of their invasive ability. In addition, bioinformatics software predicted that miR‑125b was involved in the regulation of several pathways associated with cancer, including the transforming growth factor‑β, Wnt and mitogen‑activated protein kinase signaling pathways. These data indicated for the first time, to the best of our knowledge, that miR‑125b may function as an oncogene in lung cancer.
本研究旨在探讨微小RNA（miR）-125b对肺癌细胞的生物学功能。使用miR基因芯片阵列检测95D肺癌细胞与16个人类支气管上皮（HBE）细胞之间miR的差异表达。与相邻组织相比，在细胞系和肺癌组织中观察到miR-125b的过表达，通过逆转录定量聚合酶链反应得以证实。还对miR-125b进行了生物信息学分析，包括靶标预测、基因本体论和通路分析。MTT法、流式细胞术和Transwell实验也用于检测下调miR-125b对95D细胞增殖、凋亡、侵袭能力和细胞周期的影响。与16HBE细胞相比，95D细胞中45种miR的表达存在显著差异，并且与16HBE细胞相比，95D细胞中miR-125b的表达显著更高，与相邻组织相比，肺癌组织中miR-125b的表达也显著更高。此外，抑制95D细胞中miR-125b的表达可诱导细胞凋亡、G1/S期阻滞并降低其侵袭能力。此外，生物信息学软件预测miR-125b参与调控多种与癌症相关的通路，包括转化生长因子-β、Wnt和丝裂原活化蛋白激酶信号通路。据我们所知，这些数据首次表明miR-125b在肺癌中可能作为一种癌基因发挥作用。
