Shi Xiujuan, Chen Xianzhen, Peng Hu, Song E, Zhang Ting, Zhang Junxiang, Li Jing, Swa Himaya, Li Yongxin, Kim Sekwon, Liu Xiaoqing, Zhang Chen
Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu 242000, P.R. China.
Mol Med Rep. 2015 May;11(5):3533-8. doi: 10.3892/mmr.2015.3175. Epub 2015 Jan 9.
Spindle and kinetochore‑associated protein 1 (SKA1) is an important component of the human kinetochore, which plays a key role in mitosis. The resent study was designed to investigate the role of SKA1 in human glioblastoma. The results of the present study demonstrated that SKA1 was expressed in human glioblastoma cells. In addition, the knockdown of SKA1 expression in the A172 and U251 human glioblastoma cell lines was accomplished using a lentivirus infection method. An MTT assay demonstrated that downregulation of SKA1 may inhibit cell proliferation, without affecting the cell cycle. Furthermore, knockdown of SKA1 expression resulted in reduced cell invasion. The results of the present study indicated that SKA1 may be a potential target protein for antiproliferative and anti‑invasive therapeutic strategies of human glioblastoma.
纺锤体和动粒相关蛋白1（SKA1）是人类动粒的重要组成部分，在有丝分裂中起关键作用。本研究旨在探讨SKA1在人类胶质母细胞瘤中的作用。本研究结果表明，SKA1在人类胶质母细胞瘤细胞中表达。此外，通过慢病毒感染方法实现了A172和U251人类胶质母细胞瘤细胞系中SKA1表达的敲低。MTT分析表明，SKA1的下调可能抑制细胞增殖，而不影响细胞周期。此外，SKA1表达的敲低导致细胞侵袭减少。本研究结果表明，SKA1可能是人类胶质母细胞瘤抗增殖和抗侵袭治疗策略的潜在靶蛋白。
