Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Int J Mol Med. 2019 Nov;44(5):1971-1978. doi: 10.3892/ijmm.2019.4343. Epub 2019 Sep 17.
Spindle and kinetochore‑associated protein 1 (SKA1), a microtubule‑binding subcomplex of the outer kinetochore, is essential for complete chromosomal separation. SKA1 has been suggested as a potential biomarker for various types of cancer. However, the exact role of SKA1 in esophageal squamous cell carcinoma (ESCC) remains unclear. The present study investigated whether SKA1 affects the biological behavior of ESCC. The expression of SKA1 in ESCC tissues was measured using immunohistochemistry and reverse transcription‑quantitative polymerase chain reaction. In addition, a SKA1‑silencing lentivirus was constructed, which was transfected into TE‑1 cells to establish stable SKA1‑knockdown TE‑1 cells. Proliferation was analyzed using a Celigo image cytometer and a MTS assay. Cell cycle progression and apoptosis were analyzed by flow cytometry, while cell migration was assessed using a Transwell assay. SKA1 was significantly overexpressed in ESCC tissues, and SKA1 overexpression was significantly associated with differentiation, pathological N stage and pathological tumor‑node‑metastasis stage. SKA1 was determined to be an independent prognostic factor for ESCC. Furthermore, SKA1 was significantly overexpressed in ESCC cells, and SKA1‑silencing inhibited cell proliferation and migration, arrested the cell cycle and promoted cell apoptosis. In summary, SKA1 may serve as a potential therapeutic target and prognostic biomarker for ESCC.
纺锤体和着丝粒相关蛋白 1（SKA1）是外着丝粒的微管结合亚基复合物，对于完全的染色体分离是必需的。SKA1 已被提议作为各种类型癌症的潜在生物标志物。然而，SKA1 在食管鳞状细胞癌（ESCC）中的确切作用仍不清楚。本研究旨在探讨 SKA1 是否影响 ESCC 的生物学行为。采用免疫组织化学和逆转录-定量聚合酶链反应检测 ESCC 组织中 SKA1 的表达。此外，构建了 SKA1 沉默慢病毒，转染 TE-1 细胞以建立稳定的 SKA1 敲低 TE-1 细胞。使用 Celigo 图像细胞仪和 MTS 测定法分析增殖。通过流式细胞术分析细胞周期进展和细胞凋亡，通过 Transwell 测定法评估细胞迁移。SKA1 在 ESCC 组织中显著过表达，并且 SKA1 过表达与分化、病理 N 分期和病理肿瘤-淋巴结-转移分期显著相关。SKA1 被确定为 ESCC 的独立预后因素。此外，SKA1 在 ESCC 细胞中显著过表达，并且 SKA1 沉默抑制细胞增殖和迁移，使细胞周期停滞并促进细胞凋亡。总之，SKA1 可能成为 ESCC 的潜在治疗靶点和预后生物标志物。
