环磷酸腺苷（cAMP）和钙作为富含轴膜和富含髓磷脂的膜组分刺激培养的雪旺细胞增殖的潜在介质。

Cyclic AMP and calcium as potential mediators of stimulation of cultured Schwann cell proliferation by axolemma-enriched and myelin-enriched membrane fractions.

作者信息

Meador-Woodruff J H, Lewis B L, DeVries G H

出版信息

Biochem Biophys Res Commun. 1984 Jul 18;122(1):373-80. doi: 10.1016/0006-291x(84)90485-6.

Abstract

The roles of cyclic AMP and calcium in the transduction of the mitogenic effects of central nervous system axolemma and myelin-enriched fractions on cultured Schwann cells were examined. Cyclic AMP levels were not elevated in axolemma or myelin-stimulated Schwann cells, but were increased when stimulated with cholera toxin, an adenyl cyclase activator. The mitogenicity of axolemma and myelin was markedly reduced by 2.5 mM citrate, a calcium chelator, and 10 uM trifluoroperazine, an inhibitor of calmodulin. Treatment of Schwann cells with several tumor-promoting phorbol esters caused significant enhancement of the mitogenicity of the axolemma and myelin preparations. These data suggest that the mitogenic effects of axolemma and myelin are not mediated by cyclic AMP, but may be mediated by calcium ions.

摘要

研究了环磷酸腺苷（cAMP）和钙在中枢神经系统轴突膜及富含髓磷脂的组分对培养的雪旺细胞促有丝分裂作用转导中的作用。轴突膜或髓磷脂刺激的雪旺细胞中cAMP水平未升高，但用霍乱毒素（一种腺苷酸环化酶激活剂）刺激时cAMP水平升高。2.5 mM柠檬酸盐（一种钙螯合剂）和10 μM三氟拉嗪（一种钙调蛋白抑制剂）可显著降低轴突膜和髓磷脂的促有丝分裂活性。用几种促肿瘤的佛波酯处理雪旺细胞可显著增强轴突膜和髓磷脂制剂的促有丝分裂活性。这些数据表明，轴突膜和髓磷脂的促有丝分裂作用不是由cAMP介导的，而是可能由钙离子介导的。