Colsky A S, Peacock J S
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33136.
J Immunol Methods. 1989 Nov 30;124(2):179-87. doi: 10.1016/0022-1759(89)90351-7.
The observation that exogenously supplied agents can bypass intrinsic recognition mechanisms and facilitate cellular conjugation has led to valuable insights into the mechanisms of cell function. A common feature of currently available cellular conjugation agents is their reliance on endogenous membrane molecules on both cell types as anchors for cellular interactions. In this report, we describe a method for incorporating palmitate-derivatized antibody molecules onto cell membranes where they function as 'surrogate receptors' (SR) for mediating specific cellular interactions. In this system, SR are attached to the plasma membrane by insertion of the palmitate hydrocarbon chains into the outer leaflet of the phospholipid bilayer. Therefore, the palmitate anchor bypasses the requirement for FcR or other endogenous membrane proteins in antibody-dependent cellular conjugation. Due to this mode of attachment, which is similar to that of phosphatidylinositol (PI) anchored proteins, SR-mediated cellular interactions are likely to be reminiscent of native receptor-induced conjugation, enabling SR to cooperate with endogenous target recognition structures in receptor-ligand interactions at the cell-cell interface.
外源性提供的试剂能够绕过内在识别机制并促进细胞结合，这一观察结果为深入了解细胞功能机制提供了宝贵的见解。目前可用的细胞结合试剂的一个共同特点是它们依赖于两种细胞类型上的内源性膜分子作为细胞相互作用的锚定物。在本报告中，我们描述了一种将棕榈酸酯衍生的抗体分子整合到细胞膜上的方法，在那里它们作为“替代受体”（SR）发挥作用，介导特定的细胞相互作用。在这个系统中，SR通过将棕榈酸烃链插入磷脂双分子层的外叶而附着在质膜上。因此，棕榈酸酯锚定绕过了抗体依赖性细胞结合中对FcR或其他内源性膜蛋白的需求。由于这种附着方式类似于磷脂酰肌醇（PI）锚定蛋白的附着方式，SR介导的细胞相互作用可能类似于天然受体诱导的结合，使SR能够在细胞 - 细胞界面的受体 - 配体相互作用中与内源性靶标识别结构协同作用。
