Yang Jie, Yu Xiao-Xiao, Abulaiti Abduhaer, Fei Jian-Chun
Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
Department of Pediatrics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
Exp Ther Med. 2015 Feb;9(2):543-546. doi: 10.3892/etm.2014.2121. Epub 2014 Dec 8.
The aim of the present study was to investigate the correlation between nuclear factor-κB (NF-κB) activity and pulmonary artery pressure in the pulmonary artery endothelial cells of high pulmonary blood flow rat models. A total of 50 four-week-old male Wistar rats were randomly divided into four groups: Surgery shunt group (Tn, n=15); surgery + pyrollidine dithiocarbamate (PDTC) administration group (Ti, n=15); sham control group (Co, n=10) and negative control group (Cn, n=10). The 30 rats of the Ti and Tn groups underwent carotid artery-external jugular vein anastomosis; the 15 rats in the Ti group were injected with PDTC intraperitoneally 1 h prior to surgery for a two-week continuous infusion. After 12 weeks of feeding , right ventricular systolic pressure and NF-κB activity in the pulmonary artery endothelial cells of the rats were measured. The NF-κB activity of the Tn group was significantly higher than that of the Cn group (P<0.01) and the NF-κB activity of the Ti group was lower than that of the Cn group (P<0.01); however, no significant difference was observed between the Co and Cn groups. The increased activity of NF-κB was an important factor in the pulmonary vasoconstriction and structural remodeling of rats with high pulmonary blood flow.
本研究的目的是探讨高肺血流量大鼠模型肺动脉内皮细胞中核因子-κB（NF-κB）活性与肺动脉压力之间的相关性。将50只四周龄雄性Wistar大鼠随机分为四组：手术分流组（Tn，n = 15）；手术 + 吡咯烷二硫代氨基甲酸盐（PDTC）给药组（Ti，n = 15）；假手术对照组（Co，n = 10）和阴性对照组（Cn，n = 10）。Ti组和Tn组的30只大鼠进行颈动脉-颈外静脉吻合术；Ti组的15只大鼠在手术前1小时腹腔注射PDTC，持续输注两周。饲养12周后，测量大鼠肺动脉内皮细胞的右心室收缩压和NF-κB活性。Tn组的NF-κB活性显著高于Cn组（P < 0.01），Ti组的NF-κB活性低于Cn组（P < 0.01）；然而，Co组和Cn组之间未观察到显著差异。NF-κB活性增加是高肺血流量大鼠肺血管收缩和结构重塑的重要因素。
