Chen Xiaoshuang, Li Gaofeng, Ma Shangfang, Hu Xujia
Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, P.R. China.
The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, 650118 Kunming, P.R. China.
J Chromatogr Sci. 2015 Jul;53(6):979-85. doi: 10.1093/chromsci/bmu209. Epub 2015 Jan 9.
A simple, sensitive and rapid centrifugation-assisted solid-phase extraction (SPE) with high-performance liquid chromatography (SPE-HPLC) method was developed for simultaneous determination of the metabolites loureirin A and loureirin B from Dragon's blood in rat plasma and urine. The development of the extraction procedure included optimization of some important extraction phases. After evaluation, the metabolites of Dragon's blood were extracted by centrifugation-assisted SPE and separated by using HPLC. This method showed good linearity (r(2) > 0.99), and in the rat plasma and urine, the recoveries were 93.1 and 95.7% for loureirin A and were 90.1 and 94.2% for loureirin B. The relative standard deviation (RSD) values of intraday and interday precision in rat plasma and urine for loureirin A were <3.84 and 2.01%, respectively. The RSD values of the intraday and interday precision in rat plasma and urine for loureirin B were below 4.25 and 5.83%, respectively. Thus, the established method is suitable for metabolism studies of loureirin A and loureirin B in rat plasma and urine.
建立了一种简单、灵敏、快速的离心辅助固相萃取-高效液相色谱法（SPE-HPLC），用于同时测定大鼠血浆和尿液中血竭的代谢产物龙血素A和龙血素B。萃取方法的开发包括对一些重要萃取阶段的优化。经评估，血竭代谢产物采用离心辅助SPE萃取，并用HPLC分离。该方法线性良好（r(2)>0.99），在大鼠血浆和尿液中，龙血素A的回收率分别为93.1%和95.7%，龙血素B的回收率分别为90.1%和94.2%。大鼠血浆和尿液中龙血素A日内和日间精密度的相对标准偏差（RSD）值分别<3.84%和2.01%。大鼠血浆和尿液中龙血素B日内和日间精密度的RSD值分别低于4.25%和5.83%。因此，所建立的方法适用于大鼠血浆和尿液中龙血素A和龙血素B的代谢研究。
