Vuong Tram Thu, Reine Trine M, Sudworth Amanda, Jenssen Trond G, Kolset Svein O
Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (TTV, TMR, SOK)
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (AS)
J Histochem Cytochem. 2015 Apr;63(4):280-92. doi: 10.1369/0022155415568995. Epub 2015 Jan 9.
Syndecans are important cell surface proteoglycans with many functions; yet, they have not been studied to a very large extent in primary human endothelial cells. The purpose of this study was to investigate syndecan-4 expression in cultured human umbilical vein endothelial cells (HUVECs) and assess its role in inflammatory reactions and experimental wound healing. qRT-PCR analysis revealed that syndecan-3 and syndecan-4 were highly expressed in HUVECs, whereas the expression of syndecan-1 and -2 was low. HUVECs were cultured with the inflammatory mediators lipopolysaccharide (LPS) and interleukin 1β (IL-1β). As a result, syndecan-4 expression showed a rapid and strong increase. Syndecan-1 and -2 expressions decreased, whereas syndecan-3 was unaffected. Knockdown of syndecan-4 using siRNA resulted in changes in cellular morphology and focal adhesion sites, delayed wound healing and tube formation, and increased secretion of the pro-inflammatory and angiogenic chemokine, CXCL8. These data suggest functions for syndecan-4 in inflammatory reactions, wound healing and angiogenesis in primary human endothelial cells.
Syndecans是具有多种功能的重要细胞表面蛋白聚糖；然而，它们在原代人内皮细胞中的研究程度还不是很高。本研究的目的是调查syndecan-4在培养的人脐静脉内皮细胞（HUVECs）中的表达，并评估其在炎症反应和实验性伤口愈合中的作用。qRT-PCR分析显示，syndecan-3和syndecan-4在HUVECs中高表达，而syndecan-1和-2的表达较低。用炎症介质脂多糖（LPS）和白细胞介素1β（IL-1β）培养HUVECs。结果，syndecan-4的表达迅速且强烈增加。Syndecan-1和-2的表达降低，而syndecan-3不受影响。使用siRNA敲低syndecan-4导致细胞形态和粘着斑位点发生变化，伤口愈合和管形成延迟，并增加促炎和促血管生成趋化因子CXCL8的分泌。这些数据表明syndecan-4在原代人内皮细胞的炎症反应、伤口愈合和血管生成中发挥作用。
