Manning Thomas, Mikula Rachel, Wylie Greg, Phillips Dennis, Jarvis Jackie, Zhang Fengli
Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
Bioorg Med Chem Lett. 2015 Feb 1;25(3):451-8. doi: 10.1016/j.bmcl.2014.12.053. Epub 2014 Dec 23.
The bacterium responsible for tuberculosis is increasing its resistance to antibiotics resulting in new multidrug-resistant Mycobacterium tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study, several analytical techniques including NMR, FT-ICR, MALDI-MS, LC-MS and UV/Vis are used to study the copper-Rifampicin-Polyethylene glycol (PEG-3350) complex. The copper (II) cation is a carrier for the antibiotic Rifampicin as well as nutrients for the bacterium. The NIH-NIAID cell line containing several Tb strains (including antibiotic resistant strains) is tested against seven copper-PEG-RIF complex variations.
导致结核病的细菌对抗生素的耐药性正在增强，从而产生了新的耐多药结核分枝杆菌（MDR-TB）和广泛耐药结核病（XDR-TB）。在本研究中，使用了包括核磁共振（NMR）、傅里叶变换离子回旋共振（FT-ICR）、基质辅助激光解吸电离质谱（MALDI-MS）、液相色谱-质谱联用（LC-MS）和紫外/可见光谱（UV/Vis）在内的多种分析技术来研究铜-利福平-聚乙二醇（PEG-3350）复合物。铜（II）阳离子是抗生素利福平的载体，也是细菌的营养物质。含有多种结核菌株（包括耐药菌株）的美国国立卫生研究院-美国国立过敏和传染病研究所（NIH-NIAID）细胞系针对七种铜-聚乙二醇-利福平（copper-PEG-RIF）复合物变体进行了测试。
