梅氏多锯鲉毒液中C型凝集素的鉴定
Identification of C-type isolectins in the venom of the scorpionfish Scorpaena plumieri.
作者信息
Andrich F, Richardson M, Naumann G B, Cordeiro M N, Santos A V, Santos D M, Oliveira J S, de Lima M E, Figueiredo S G
机构信息
Departamento de Ciências Fisiológicas, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, Vitória, ES 29043-900, Brazil; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil.
Diretoria do Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Rua Conde Pereira Carneiro, 80, Gameleira, Belo Horizonte, MG 30510-010, Brazil.
出版信息
Toxicon. 2015 Mar;95:67-71. doi: 10.1016/j.toxicon.2015.01.004. Epub 2015 Jan 7.
Chemical analyses of the hemagglutinating fraction from Scorpaena plumieri venom revealed that it contains five components (Sp-CL 1-5) with similar chromatographic elution profiles (35-38% of acetonitrile), molecular masses (16,800-17,000 Da) and N-terminal sequences, suggesting that they are isoforms of the same protein. The amino acid sequence of Sp-CL4 was determined and shown to have homology with fish C-type lectins. These data demonstrate for the first time the presence of C-type isolectins in a scorpionfish venom.
对梅氏多线鱼毒液中血凝活性成分的化学分析表明，它含有五种成分（Sp-CL 1-5），这些成分具有相似的色谱洗脱图谱（35-38% 的乙腈）、分子量（16,800-17,000 Da）和 N 端序列，这表明它们是同一种蛋白质的同工型。Sp-CL4 的氨基酸序列已被确定，并且显示出与鱼类 C 型凝集素具有同源性。这些数据首次证明了鲉鱼毒液中存在 C 型异凝集素。
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