超高效液相色谱法与高效液相色谱法测定维生素 C 的比较

Comparison of UPLC and HPLC methods for determination of vitamin C.

机构信息

The Poznań University of Economics, Faculty of Commodity Science, al. Niepodległości 10, 61-875 Poznań, Poland.

出版信息

Food Chem. 2015 May 15;175:100-5. doi: 10.1016/j.foodchem.2014.11.104. Epub 2014 Nov 26.

DOI:10.1016/j.foodchem.2014.11.104

Abstract

Ultra performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods for determination of ascorbic acid (AA) and total AA (TAA) contents (as the sum of AA and dehydroascorbic acid (DHAA) after its reduction to AA) in fruit beverages and in pharmaceutical preparations were compared. Both methods are rapid: total time of analysis was 15 and 6 min for HPLC and UPLC methods, respectively. The methods were validated in terms of linearity, instrument precision, limits of detection (LOD) and quantification (LOQ), accuracy and recovery. Intra- and inter-day instrument precisions for fruit juices, expressed as RSD, were 2.2% and 2.4% for HPLC, respectively, and 1.7% and 1.9% for UPLC, respectively. For vitamin C tablets, inter- and intra-day precisions were 0.4% and 0.5%, respectively (HPLC), and 0.5% and 0.3%, respectively (UPLC). Both methods were sensitive: LOD was 0.049 μg/mL for HPLC and 0.024 μg/mL for UPLC while LOQs were 0.149 and 0.073 μg/mL for HPLC and UPLC, respectively. These methods could be useful in the routine qualitative and quantitative analysis of AA or TAA in pharmaceutical preparations or fruit beverages. However, UPLC method is more sensitive, faster and consumes less eluent.

摘要

超高效液相色谱（UPLC）和高效液相色谱（HPLC）法测定水果饮料和药物制剂中抗坏血酸（AA）和总 AA（TAA）含量（AA 和脱氢抗坏血酸（DHAA）还原为 AA 后的总和）进行了比较。这两种方法都很快：HPLC 和 UPLC 方法的总分析时间分别为 15 分钟和 6 分钟。从线性、仪器精密度、检测限（LOD）和定量限（LOQ）、准确性和回收率等方面对方法进行了验证。果汁的日内和日间仪器精密度分别表示为 HPLC 的 RSD 为 2.2%和 2.4%，UPLC 分别为 1.7%和 1.9%。对于维生素 C 片剂，HPLC 的日间和日内精密度分别为 0.4%和 0.5%，UPLC 分别为 0.5%和 0.3%。这两种方法都很灵敏：HPLC 的 LOD 为 0.049 μg/mL，UPLC 的 LOD 为 0.024 μg/mL，而 HPLC 和 UPLC 的 LOQs 分别为 0.149 和 0.073 μg/mL。这些方法可用于药物制剂或水果饮料中 AA 或 TAA 的常规定性和定量分析。然而，UPLC 方法更灵敏、更快且消耗的洗脱液更少。