Sun Hui, Lai Jia-Ping, Chen Fang, Zhu De-Rong
College of Environmental Science & Engineering, Guangzhou University, Guangzhou, 510006, China.
Anal Bioanal Chem. 2015 Feb;407(6):1745-52. doi: 10.1007/s00216-014-8420-5. Epub 2015 Jan 11.
A simple, fast, and universal suspension polymerization method was used to synthesize the molecularly imprinted microspheres (MIMs) for the topical anesthetic benzocaine (BZC). The desired diameter (10-20 μm) and uniform morphology of the MIMs were obtained easily by changing one or more of the synthesis conditions, including type and amount of surfactant, stirring rate, and ratio of organic to water phase. The MIMs obtained were used as a molecular-imprinting solid-phase-extraction (MISPE) material for extraction of BZC in human serum and fish tissues. The MISPE results revealed that the BZC in these biosamples could be enriched effectively after the MISPE operation. The recoveries of BZC on MIMs cartridges were higher than 90% (n = 3). Finally, an MISPE-HPLC method with UV detection was developed for highly selective extraction and fast detection of trace BZC in human serum and fish tissues. The developed method could also be used for the enrichment and detection of BZC in other complex biosamples.
采用一种简单、快速且通用的悬浮聚合法合成了用于局部麻醉剂苯佐卡因（BZC）的分子印迹微球（MIMs）。通过改变一种或多种合成条件，包括表面活性剂的类型和用量、搅拌速率以及有机相和水相的比例，可以轻松获得所需直径（10 - 20μm）且形态均匀的MIMs。所制备的MIMs用作分子印迹固相萃取（MISPE）材料，用于从人血清和鱼组织中萃取BZC。MISPE结果表明，经过MISPE操作后，这些生物样品中的BZC能够得到有效富集。BZC在MIMs柱上的回收率高于90%（n = 3）。最后，建立了一种具有紫外检测的MISPE - HPLC方法，用于高选择性萃取和快速检测人血清和鱼组织中的痕量BZC。所建立的方法还可用于其他复杂生物样品中BZC的富集和检测。
