Dayaram Anisha, Potter Kristen A, Pailes Roberta, Marinov Milen, Rosenstein Dana D, Varsani Arvind
School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand.
School of Earth Sciences and Environmental Sustainability, Northern Arizona University, Flagstaff, AZ 86011, USA.
Infect Genet Evol. 2015 Mar;30:278-287. doi: 10.1016/j.meegid.2014.12.037. Epub 2015 Jan 9.
Next generation sequencing and metagenomic approaches are commonly used for the identification of circular replication associated protein (Rep)-encoding single stranded (CRESS) DNA viruses circulating in various environments. These approaches have enabled the discovery of some CRESS DNA viruses associated with insects. In this study we identified and recovered 31 viral genomes which represent 24 distinct CRESS DNA viruses from seven dragonfly species (Rhionaeschna multicolor, Erythemis simplicicollis, Erythrodiplax fusca, Libellula quadrimaculata, Libellula saturata, Pachydiplax longipennis, and Pantala hymenaea) and two damselfly species (Ischnura posita, Ischnura ramburii) sampled in various locations in the states of Arizona and Oklahoma of the United States of America (USA). We also identified Sclerotinia sclerotiorum hypovirulence-associated DNA virus-1 (SsHADV-1) in P. hymenaea, E. simplicicollis and I. ramburii sampled in Oklahoma, which is the first report of SsHADV-1 in the New World. The genome architectures of the CRESS DNA viruses recovered vary, but they all have at least two major open reading frames (ORFs) that have either a bidirectional or unidirectional arrangement. Four of the viral genomes recovered, in addition to the three isolates of SsHADV-1, show similarities to viruses of the proposed gemycircularvirus group. Analysis of the Rep encoded by the remaining 24 viral genomes reveals that these are highly diverse and allude to the fact that they represent novel CRESS DNA viruses.
下一代测序和宏基因组学方法通常用于鉴定在各种环境中传播的编码环状复制相关蛋白（Rep）的单链（CRESS）DNA病毒。这些方法使得发现了一些与昆虫相关的CRESS DNA病毒。在本研究中，我们从美国亚利桑那州和俄克拉荷马州不同地点采集的七种蜻蜓物种（多色大蜓、纯色赤蜻、褐黄赤蜻、四斑丽翅蜻、饱和丽翅蜻、长腹蜻和斑痣细蟌）和两种豆娘物种（波氏细蟌、兰氏细蟌）中鉴定并获得了31个病毒基因组，它们代表24种不同的CRESS DNA病毒。我们还在俄克拉荷马州采集的斑痣细蟌、纯色赤蜻和波氏细蟌中鉴定出了核盘菌低毒力相关DNA病毒1（SsHADV-1），这是该病毒在新大陆的首次报道。所获得的CRESS DNA病毒的基因组结构各不相同，但它们都至少有两个主要的开放阅读框（ORF），其排列方式为双向或单向。除了三个SsHADV-1分离株外，所获得的四个病毒基因组与拟议的环质病毒属病毒具有相似性。对其余24个病毒基因组编码的Rep的分析表明，它们高度多样，并暗示它们代表新型的CRESS DNA病毒。
