Chrzastek Klaudia, Kraberger Simona, Schmidlin Kara, Fontenele Rafaela S, Kulkarni Arun, Chappell Len, Dufour-Zavala Louise, Kapczynski Darrell R, Varsani Arvind
Exotic and Emerging Avian Viral Disease Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, USDA, 934 College Station Road, Athens, GA 30605, USA.
The Biodesign Center of Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, 1001 S. McAllister Ave, Tempe, AZ 85287, USA.
Microorganisms. 2021 Dec 16;9(12):2602. doi: 10.3390/microorganisms9122602.
High-throughput sequencing approaches offer the possibility to better understand the complex microbial communities associated with animals. Viral metagenomics has facilitated the discovery and identification of many known and unknown viruses that inhabit mucosal surfaces of the body and has extended our knowledge related to virus diversity. We used metagenomics sequencing of chicken buccal swab samples and identified various small DNA viruses with circular genome organization. Out of 134 putative circular viral-like circular genome sequences, 70 are cressdnaviruses and 26 are microviruses, whilst the remaining 38 most probably represent sub-genomic molecules. The cressdnaviruses found in this study belong to the , and families as well as previously described CRESS1 and naryavirus groups. Among these, genomoviruses and smacoviruses were the most prevalent across the samples. Interestingly, we also identified 26 bacteriophages that belong to the family, whose members are known to infect enterobacteria.
高通量测序方法为更好地了解与动物相关的复杂微生物群落提供了可能。病毒宏基因组学促进了对许多存在于身体黏膜表面的已知和未知病毒的发现与鉴定，并扩展了我们对病毒多样性的认识。我们对鸡口腔拭子样本进行了宏基因组测序，鉴定出了各种具有环状基因组结构的小型DNA病毒。在134个假定的类病毒环状基因组序列中，70个是十字花科病毒，26个是微小病毒，其余38个很可能代表亚基因组分子。本研究中发现的十字花科病毒属于 、 和 科以及先前描述的CRESS1和纳拉病毒组。其中，基因组病毒和小病毒在样本中最为普遍。有趣的是，我们还鉴定出了26种属于 科的噬菌体，其成员已知可感染肠道细菌。
