Guerriero E, Accardo M, Capone F, Colonna G, Castello G, Costantini S
IRCCS Istituto Nazionale Tumori.
Eur J Histochem. 2014 Dec 1;58(4):2433. doi: 10.4081/ejh.2014.2433.
Selenium is an essential trace mineral of fundamental importance to human healthy and exerts its biological function through selenoproteins. In particular, Selenoprotein M (SELM) is located in the endoplasmic reticulum and contains the common redox motif of cysteine-X-X-selenocysteine type. It attracts great attention due to its high expression in brain and its potential roles as antioxidant, neuroprotective, and cytosolic calcium regulator. Recently, our group found SELM over-expression in human hepatocellular carcinoma (HCC) cell lines. In this report some paraffin-embedded tissues from liver biopsy of patients with hepatitis C virus (HCV)-related cirrhosis and HCC were immunohistochemically stained and SELM expression scoring was evaluated. Our results evidence for the first time an increase of SELM expression in HCC liver tissues, and its gradual expression raise associated with an increased malignancy grade. Therefore, we propose to use i) SELM as putative marker for HCC as well as ii) simple immunohistochemistry technique to distinguish between the different grades of malignancy.
硒是一种对人体健康至关重要的必需微量元素，它通过硒蛋白发挥其生物学功能。特别是，硒蛋白M（SELM）位于内质网中，含有半胱氨酸-X-X-硒代半胱氨酸类型的常见氧化还原基序。由于其在大脑中的高表达以及作为抗氧化剂、神经保护剂和胞质钙调节剂的潜在作用，它引起了极大的关注。最近，我们小组发现SELM在人肝癌（HCC）细胞系中过表达。在本报告中，对一些来自丙型肝炎病毒（HCV）相关肝硬化和HCC患者肝活检的石蜡包埋组织进行免疫组织化学染色，并评估SELM表达评分。我们的结果首次证明HCC肝组织中SELM表达增加，并且其表达逐渐升高与恶性程度增加相关。因此，我们建议使用i）SELM作为HCC的推定标志物，以及ii）简单的免疫组织化学技术来区分不同等级的恶性肿瘤。
