通过定向进化改进和重新利用生物催化剂。
Improving and repurposing biocatalysts via directed evolution.
作者信息
Denard Carl A, Ren Hengqian, Zhao Huimin
机构信息
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States; Department of Chemistry, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States; Department of Biochemistry, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States; Department of Bioengineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
出版信息
Curr Opin Chem Biol. 2015 Apr;25:55-64. doi: 10.1016/j.cbpa.2014.12.036. Epub 2015 Jan 8.
Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.
在过去二十年中，定向进化已成为蛋白质工程的一项主要技术，并开创了工业生物催化的新时代。定向进化不仅提供了提高已知生物催化剂活性的工具，还赋予了生物催化剂自然界中未曾出现过的化学反应活性。在此，我们将讨论过去两年间在提高生物催化剂的热稳定性、立体选择性和活性以及创造新型酶方面所取得的最新成果。
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