Qian Yu, Li Gui-Jie, Wang Rui, Zhou Ya-Lin, Sun Peng, Zhao Xin
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China.
J Cancer Res Ther. 2014 Oct-Dec;10(4):1045-51. doi: 10.4103/0973-1482.137979.
Insect tea is widely used a traditional drink or traditional Chinese medicine in China. This study was conducted with an aim to determine the in vitro anticancer effect of Insect tea in cancer cells.
The anticancer effects of Insect tea were evaluated in human tongue carcinoma TCA8113 cells using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry analysis, nuclear staining with 4,6-diamidino-2-phenylindole (DAPI), reverse transcription-polymerase chain reaction (RT-PCR) analysis, and western bolt assay.
At 200 μg/mL, Insect tea inhibited the growth of TCA8113 cells by 80.7%, which was higher than the inhibition caused by 100 μg/mL Insect tea but lower than that of 200 μg/mL green tea. Compared to the control cancer cells, Insect tea significantly (P<0.05) induced apoptosis as determined by DAPI staining and flow cytometry analysis results. Insect tea significantly induced apoptosis in cancer cells by upregulating BAX, CASP3, CASP9 and downregulating BCL2. Genes encoding nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were significantly downregulated by Insect tea, demonstrating its anti-inflammatory properties. Insect tea also exerted a great anti-metastasis effect on cancer cells as demonstrated by decreased expression of matrix metalloproteinase (MMP) genes and increased expression of tissue inhibitors of metalloproteinases (TIMPs).
The results showed that Insect tea has good in vitro anticancer effects in TCA8113 cells, like green tea.
虫茶在中国作为传统饮品或中药被广泛使用。本研究旨在确定虫茶对癌细胞的体外抗癌作用。
使用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴盐（MTT）法、流式细胞术分析、4,6-二脒基-2-苯基吲哚（DAPI）核染色、逆转录-聚合酶链反应（RT-PCR）分析和蛋白质免疫印迹法，评估虫茶对人舌癌TCA8113细胞的抗癌作用。
在200μg/mL时，虫茶对TCA8113细胞生长的抑制率为80.7%，高于100μg/mL虫茶的抑制率，但低于200μg/mL绿茶的抑制率。与对照癌细胞相比，根据DAPI染色和流式细胞术分析结果，虫茶显著（P<0.05）诱导细胞凋亡。虫茶通过上调BAX、CASP3、CASP9和下调BCL2，显著诱导癌细胞凋亡。虫茶显著下调编码活化B细胞的核因子κB轻链增强子（NF-κB）、诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的基因，证明其具有抗炎特性。虫茶还对癌细胞具有显著的抗转移作用，表现为基质金属蛋白酶（MMP）基因表达降低和金属蛋白酶组织抑制剂（TIMPs）表达增加。
结果表明，虫茶在体外对TCA8113细胞具有良好的抗癌作用，与绿茶类似。
